Müller, Erik Sosedov, Olga Gröning, Janosch Alexander David Stolz, Andreas
Published in
Biotechnology letters
Chiral 2-hydroxycarboxylic acids and 2-hydroxycarboxamides are valuable synthons for the chemical industry. The biocatalytic syntheses of (R)-mandelic acid and (R)-mandelic acid amide by recombinant Escherichia coli clones were studied. Strains were constructed which simultaneously expressed a (R)-specific oxynitrilase (hydroxynitrile lyase) from t...
Li, Kangning Varden, Lara Henderson, Althea Lufkin, Thomas Kraus, Petra
Published in
Biotechnology letters
Interactions of cells with their neighbors and influences by the surrounding extracellular matrix (ECM) is reflected in a cells transcriptome and proteome. In tissues comprised of heterogeneous cell populations or cells depending on ECM signalling cues such as those of the intervertebral disc (IVD), this information is obscured or lost when cells a...
Nosheen, Shaista Yang, Junhuan Naz, Tahira Nazir, Yusuf Ahmad, Muhammad Ijaz Fazili, Abu Bakr Ahmad Li, Shaoqi Mustafa, Kiren Song, Yuanda
Published in
Biotechnology letters
AMP-activated protein kinase (AMPK) is an important regulator for lipid accumulation, potentially known to have an inhibitory role in lipid synthesis. It inactivates acetyl-CoA carboxylase (ACC), an important regulatory enzyme required for lipid synthesis. However, in Mucor circinelloides, AMPK and its association with lipid accumulation has not be...
Mistry, Harsh Thakor, Rashmi Patil, Chirag Trivedi, Jitendra Bariya, Himanshu
Published in
Biotechnology letters
To assess the extracellular synthesis of silver nanoparticles using marine derived fungi Aspergillus brunneoviolaceus with their antibacterial and antioxidant activities. The biosynthesis of silver nanoparticles was estimated by the change in color from light yellow to dark brown within 36 h as the reaction progressed. UV-Visible spectroscopy exhib...
Wu, Shimin Zhao, Peng Li, Qingyang Tian, Pingfang
Published in
Biotechnology letters
Glycerol-based biosynthesis of 3-hydroxypropionic acid (3-HP) in Klebsiella pneumoniae involves two reactions: glycerol conversion to 3-hydroxypropionaldehyde (3-HPA) by glycerol dehydratase, and 3-HPA conversion to 3-HP by aldehyde dehydrogenase (ALDH). The ALDH catalysis consumes a lot of cofactor nicotinamide adenine dinucleotide (NAD+), which c...
Kim, Sujung Na, Jungup Nie, Hualin Kim, Jiseong Lee, Jeongeun Kim, Sunhyung
Published in
Biotechnology letters
To investigate the response of Caragana microphylla in salt condition, transcriptome analysis, differentially expressed genes (DEGs) comparison with Arabidopsis thaliana, and the chlorophyll content analysis were performed. Gene Ontology (GO) term, and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses of DEGs indicated that salt condi...
Fouladiha, Hamideh Marashi, Sayed-Amir Li, Shangzhong Li, Zerong Masson, Helen O Vaziri, Behrouz Lewis, Nathan E
Published in
Biotechnology letters
Chinese hamster ovary (CHO) cells are the leading cell factories for producing recombinant proteins in the biopharmaceutical industry. In this regard, constraint-based metabolic models are useful platforms to perform computational analysis of cell metabolism. These models need to be regularly updated in order to include the latest biochemical data ...
Yu, Xijia Wei, Huiling Liu, Xianrui Liu, Dong Fan, Aili Su, Haijia
Published in
Biotechnology letters
To determine the effect of sea salt on the resistance of Trichoderma harzianum LZDX-32-08 to hygromycin B and speculate the possible mechanisms involved via transcriptome analysis. Sea salt addition in media to simulate marine environment significantly increased the tolerance of marine-derived fungus Trichoderma harzianum LZDX-32-08 to hygromycin B...
Rich, Michelle H Sharrock, Abigail V Ashoorzadeh, Amir Patterson, Adam V Smaill, Jeff B Ackerley, David F
Published in
Biotechnology letters
To use directed evolution to improve YfkO-mediated reduction of the 5-nitroimidazole PET-capable probe SN33623 without impairing conversion of the anti-cancer prodrug CB1954. Two iterations of error-prone PCR, purifying selection, and FACS sorting in a DNA damage quantifying GFP reporter strain were used to identify three YfkO variants able to sens...
Faria, Larissa Lemos Morales, Sergio Andres Villalba Prado, José Pedro Zanetti Dias, Giancarlo de Souza de Almeida, Alex Fernando Xavier, Michelle da Cunha Abreu da Silva, Elda Sabino Maiorano, Alfredo Eduardo Perna, Rafael Firmani
Published in
Biotechnology letters
Extracellular fructosyltransferase (FTase, E.C.2.4.1.9) from Aspergillus oryzae IPT-301 was immobilized on silica gel by adsorption and biochemically characterized aiming at its application in the transfructosylation reaction of sucrose for the production of fructooligossaccarides (FOS). The transfructosylation activity (AT) was maximized by the ex...
