Determination of kinetic coefficients for an acetate enrichment culture by the infinite dilution technique.
Published in Biotechnology and bioengineering
Some practical aspects of computer applications in a fermentor hall.
Published in Biotechnology and bioengineering
The use of computers in a fermentation pilot plant is described from a practical point of view. The aim is not the application of a computer to a single special process but a general application of computers to prepare and present data for the following analysis of fermentation processes. The hardware is normally bought from computer manufacturers,...
Determination of substrate concentrations by a computerized enzyme electrode.
Published in Biotechnology and bioengineering
A numerical treatment of the signal produced by an electrode onto which an artificial enzyme membrane is mounted can give the concentration of the substrate (glucose, saccharose, lactose, amino acids, etc.) in solution. In the example of a glucose analyzer, in which glucose oxidase catalyzes the oxidation of glucose, the computer receives pO(2) lev...
New concepts for rapid yeast settling. II. pH switching with an inert powder.
Published in Biotechnology and bioengineering
A new technique is outlined for the rapid settling of yeast cells in fermentation media. The technique involved the addition of dense, inert particles (nickel powder) to a yeast suspension (Saccharomyces cerevisiae) at pH 4.5 and a rapid change of pH to 8.0-9.0. When the pH was changed large flocs formed immediately and settled rapidly, leaving a c...
Fermentation monitoring by polymeric-resin-based microbiol assay.
Published in Biotechnology and bioengineering
A polymeric-resin-based microbiological assay is shown to yield results consistent with classical inhibition zone theory. This novel assay is applicable to cycloheximide, an antifungal antibiotic, at concentrations below 1.0 mug/mL and up to 800 mug/mL. Addition of ethanol to the cut-out wells of the assay plate helps to release the antibiotic and ...
Prostaglandin synthesis from arachidonic acid by immobilized ram seminal microsomes.
Published in Biotechnology and bioengineering
Continuous simultaneous saccharification and fermentation of starch using Zymomonas mobilis.
Published in Biotechnology and bioengineering
A continuous process involving simultaneous saccharification and fermentation of liquefied starch has been developed using Zymomonas mobilis. Amyloglucosidase retention and cell recycle have been effected by using an Amicon hollow-fiber membrane system with a MW cutoff of 5000. Relatively high productivities of up to 60 g L(-1) h(-1) have been achi...
The use of the inverted linear model of enzyme decay to plan a strategy for enzymatic batch reactions and to assess the ...
Published in Biotechnology and bioengineering
This article is concerned with the development of a model to plan a strategy for an enzymatic batch process where enzyme is subjected to deactivation described by the inverted linear decay model. The particular system studied is the enzymatic hydrolysis of penicillin to 6-amino penicillanic acid (6 APA), but the model can be utilized with other bat...
Kinetic and thermal characteristics of enzyme-graft copolymers.
Published in Biotechnology and bioengineering
The characteristics of horseradish peroxidase (HRP) immobilized onto Sepharose by a photochemical-initiated graft copolymerization are presented. Active copolymers were synthesized using different amounts of glycidylmethacrylate (GMA), bisacryloylpiperazine (BAP), or 1,3,5-hexhydrotriacryloyl-(s)-triazine (HTsT) as functional monomer. The activitie...
The use of chaotropic salts for separation of ribonucleic acids and proteins from yeast nucleoproteins.
Published in Biotechnology and bioengineering
The effect of chaotropic salts on the dissociation of ribonucleic acid from yeast nucleoprotein complex was studied. The effectiveness of various salts on the dissociation of ribonucleic acid followed the chaotropic series; i.e., Cl(3)CCOONa = NaClO(4) > NaBr > NaCl. Treatment of the nucleoprotein complex with 0.5M Cl(3)CCOONa or NaClO(4) resulted ...
