Sakaki, Ayumu Namatame, Tetsushi Nakaya, Makoto Omasa, Takeshi
Published in
Biotechnology and bioengineering
To enhance the robustness and flexibility of biopharmaceutical manufacturing, a paradigm shift toward methods of continuous processing, such as perfusion, and fundamental technologies for high-throughput process development are being actively investigated. The continuous upstream process must establish an advanced control strategy to ensure a "Stat...
Müller, Henri Deffur, Charlotte Schmideder, Stefan Barthel, Lars Friedrich, Tiaan Mirlach, Lukas Hammel, Jörg U Meyer, Vera Briesen, Heiko
Published in
Biotechnology and bioengineering
Filamentous fungi produce a wide range of relevant biotechnological compounds. The close relationship between fungal morphology and productivity has led to a variety of analytical methods to quantify their macromorphology. Nevertheless, only a µ-computed tomography (µ-CT) based method allows a detailed analysis of the 3D micromorphology of fungal p...
Staar, Marcel Schallmey, Anett
Published in
Biotechnology and bioengineering
Halohydrin dehalogenase HheG is an industrially interesting biocatalyst for the preparation of different β-substituted alcohols starting from bulky internal epoxides. We previously demonstrated that the immobilization of different HheG variants in the form of cross-linked enzyme crystals (CLECs) yielded stable and reusable enzyme immobilizes with i...
Fu, Qiang Lee, Yong Suk Green, Erica A Wang, Yongdan Park, So Young Polanco, Ashli Lee, Kelvin H Betenbaugh, Michael McNally, David Yoon, Seongkyu
...
Published in
Biotechnology and bioengineering
Recombinant adeno-associated virus (rAAV) vectors are a promising platform for in vivo gene therapies. However, cost-effective, well-characterized processes necessary to manufacture rAAV therapeutics are challenging to develop without an understanding of how process parameters (PPs) affect rAAV product quality attributes (PQAs). In this work, a cen...
Wang, Jiarui Chen, Jingyi Studts, Joey Wang, Gang
Published in
Biotechnology and bioengineering
Current manufacturing and development processes for therapeutic monoclonal antibodies demand increasing volumes of analytical testing for both real-time process controls and high-throughput process development. The feasibility of using Raman spectroscopy as an in-line product quality measuring tool has been recently demonstrated and promises to rel...
Anderson, Sean M Seto, Elbert Chau, David Lee, Brian Vail, Andrew Ding, Sheng Voloshin, Alexei Nagel, Mark
Published in
Biotechnology and bioengineering
The most straightforward method to increase monoclonal antibody (mAb) product yield is to complete the purification process in less steps. Here, three different fiber chromatographic devices were implemented using a holistic approach to intensify the mAb purification process and increase yield. Fiber protein A (proA) chromatography was first invest...
McRae, Oliver Walls, Peter L L Natarajan, Venkatesh Antoniou, Chris Bird, James C
Published in
Biotechnology and bioengineering
In the biotechnology industry, ensuring the health and viability of mammalian cells, especially Chinese Hamster Ovary (CHO) cells, plays a significant role in the successful production of therapeutic agents. These cells are typically cultivated in aerated bioreactors, where they encounter fluid stressors from rapidly deforming bubbles. These stress...
Guo, Xiaomeng Li, Zhibin He, Niling Zhang, Bin Liu, Xiucai Bao, Jie
Published in
Biotechnology and bioengineering
High chiral purity of lactic acid is a crucial indicator for the synthesis of chiral lactide as the primary intermediate chemical for ring-open polymerization of high molecular weight polylactic acid (PLA). Lignocellulose biomass is the most promising carbohydrate feedstock for commercial production of PLA, but the presence of trace d-lactic acid i...
Maltais, Jean-Sébastien Lord-Dufour, Simon Morasse, Audrey Stuible, Matthew Loignon, Martin Durocher, Yves
Published in
Biotechnology and bioengineering
More than half of licensed therapeutic recombinant proteins (r-proteins) are manufactured using constitutively-expressing, stably-transfected Chinese hamster ovary (CHO) clones. While constitutive CHO expression systems have proven their efficacy for the manufacturing of monoclonal antibodies, many next-generation therapeutics such as cytokines and...
Gupta, Mainak Das Flaskamp, Yannick Roentgen, Robin Juergens, Hannes Armero-Gimenez, Jorge Albrecht, Frank Hemmerich, Johannes Arfi, Zulfaquar Ahmad Neuser, Jakob Spiegel, Holger
...
Published in
Biotechnology and bioengineering
Eukaryotic cell-free protein synthesis (CFPS) can accelerate expression and high-throughput analysis of complex proteins with functionally relevant post-translational modifications (PTMs). However, low yields and difficulties scaling such systems have prevented their widespread adoption in protein research and manufacturing. Here, we provide detail...
