Ou, Jianfa Tang, Yawen Xu, Jianlin Tucci, Julian Borys, Michael C Khetan, Anurag
Published in
Biotechnology and bioengineering
Recombinant adeno-associated virus (rAAV) is rapidly emerging as the preferred delivery vehicle for gene therapies, with promising advantages in safety and efficacy. Key challenges in systemic in-vivo rAAV gene therapy applications are the gap in production capabilities versus potential market demand and complex production process. This review summ...
McKee, Jae A Olsen, Elisabet A Wills Kpeli, Gideon Brooks, Moriah R Beitollahpoor, Mohamadreza Pesika, Noshir S Burow, Matthew E Mondrinos, Mark J
Published in
Biotechnology and bioengineering
Physical characteristics of solid tumors such as dense internal microarchitectures and pathological stiffness influence cancer progression and treatment. While it is routine to engineer culture substrates and scaffolds with elastic moduli that approximate tumors, these models often fail to capture characteristic internal microarchitectures such as ...
Narayanan, Sreeja Baburajan, Adheena Panangattu Muhammad, Mumtaz Joseph, Andrea Vemula, Praveen Kumar Bhat, Sarita Ganapathy
Published in
Biotechnology and bioengineering
The potential of bacteria-based immunotherapy lies in its ability to inherently enhance immune responses. However, the “liveness” of bacteria poses risks of bacterial escape, nonspecific immuno-stimulation, and ethical concerns, limiting their acceptability in immunotherapy. In this scenario, nonliving empty bacterial-cell envelopes, named bacteria...
Mohammadzadehmarandi, Aylin Determan, Amy Krumm, Christian McIntosh, Lucas D Zydney, Andrew L
Published in
Biotechnology and bioengineering
The transition to continuous biomanufacturing has led to renewed interest in alternative approaches for downstream processing of monoclonal antibody (mAb) products. In this study, we examined the potential of using high-performance countercurrent membrane purification (HPCMP) for the removal of host cell proteins (HCPs) derived from Chinese Hamster...
Sharma, Surbhi Mahadevan, Jagadeesh Giri, Lopamudra Mitra, Kishalay
Published in
Biotechnology and bioengineering
In recent times, it has been realized that novel vaccines are required to combat emerging disease outbreaks, and faster optimization is required to respond to global vaccine demands. Although, fed-batch operations offer better productivity, experiment-based optimization of a new fed-batch process remains expensive and time-consuming. In this contex...
Xiang, Shaoxun Zhang, Jinliang Yu, Le Tian, Jun Tang, Wenxiu Tang, Hao Xu, Kecui Wang, Xin Cui, Yanyan Ren, Kaidi
...
Published in
Biotechnology and bioengineering
Intensified fed-batch (IFB), a popular cell culture intensification strategy, has been widely used for productivity improvement through high density inoculation followed by fed-batch cultivation. However, such an intensification strategy may counterproductively induce rapidly progressing cell apoptosis and difficult-to-sustain productivity. To impr...
Ma, Shuai Wang, Feiyu Xuejing, Zhang Liping, Qiao Xueping, Guo Lu, Xuemei Qi, Qingsheng
Published in
Biotechnology and bioengineering
Streptococcus thermophilus has been extensively used in industrial milk fermentation. However, lack of efficient genetic manipulation approaches greatly hampered the industrial application of this species. Here, we repurposed the endogenous CRISPR1 and CRISPR3 systems, both belong to type II-A CRISPR-Cas9, by delivering a self-targeting CRISPR arra...
Afzal, Mohammad A Zydney, Andrew L
Published in
Biotechnology and bioengineering
Virus filtration is a crucial step in ensuring the high levels of viral clearance required in the production of biotherapeutics produced in mammalian cells or derived from human plasma. Previous studies have reported that virus retention is often reduced in the presence of therapeutic proteins due to membrane fouling; however, the underlying mechan...
Serbent, Maria Pilar Magario, Ivana Saux, Clara
Published in
Biotechnology and bioengineering
Despite their high persistence in the environment, organochlorines (OC) are widely used in the pharmaceutical industry, in plastics, and in the manufacture of pesticides, among other applications. These compounds and the byproducts of their decomposition deserve attention and efficient proposals for their treatment. Among sustainable alternatives, ...
Cortada-Garcia, Joan Haggarty, Jennifer Weidt, Stefan Daly, Rónán Arnold, S Alison Burgess, Karl
Published in
Biotechnology and bioengineering
Fermentation monitoring is a powerful tool for bioprocess development and optimization. On-line metabolomics is a technology that is starting to gain attention as a bioprocess monitoring tool, allowing the direct measurement of many compounds in the fermentation broth at a very high time resolution. In this work, targeted on-line metabolomics was u...
