Huang, Yiming Wipat, Anil Bacardit, Jaume
Published in
Biotechnology and bioengineering
Foreign proteins are produced by introducing synthetic constructs into host bacteria for biotechnology applications. This process can cause resource competition between synthetic circuits and host cells, placing a metabolic burden on the host cells which may result in load stress and detrimental physiological changes. Consequently, the host bacteri...
Yang, Jizhong Tang, Jiezhang Dang, Juanli Rong, Xiangke Wang, Kai Zhang, Zhaoxiang Hou, Mengmeng Yu, Zhou Yi, Chenggang
Published in
Biotechnology and bioengineering
Recent developments in the field of regenerative surgeries and medical applications have led to a renewed interest in adipose tissue-enriched mesenchymal stem cell scaffolds. Various advantages declared for the decellularized adipose matrix (DAM) have caused its extensive use in the transfer of stem cells or growth factors for soft tissue regenerat...
Sharma, Avinash James, Abija Kapoor, Deepak N Kaurav, Hemlata Sharma, Abhishek Kumar Nagraik, Rupak
Published in
Biotechnology and bioengineering
Many of the infectious diseases are ubiquitous in nature and pose a threat to global and public health. The original cause for such type of serious maladies can be summarized as the scarcity of appropriate analysis and treatment methods. Pulmonary diseases are considered one of the life-threatening lung diseases that affect millions of people globa...
Debnath, Nabendu Yadav, Pooja Mehta, Praveen K Gupta, Priyamvada Kumar, Deepak Kumar, Ashwani Gautam, Vibhav Yadav, Ashok K
Published in
Biotechnology and bioengineering
Probiotic microorganisms have been used for therapeutic purposes for over a century, and recent advances in biotechnology and genetic engineering have opened up new possibilities for developing therapeutic approaches using indigenous probiotic microorganisms. Diseases are often related to metabolic and immunological factors, which play a critical r...
Sander, Kyle Abel, Anthony J Friedline, Skyler Sharpless, William Skerker, Jeffrey Deutschbauer, Adam Clark, Douglas S Arkin, Adam P
Published in
Biotechnology and bioengineering
Species of bacteria from the genus Cupriavidus are known, in part, for their ability to produce high amounts of poly-hydroxybutyrate (PHB) making them attractive candidates for bioplastic production. The native synthesis of PHB occurs during periods of metabolic stress, and the process regulating the initiation of PHB accumulation in these organism...
Boroujeni, Mahshid Badakhshan Mohebi, Samane Malekian, Azam Shahraeini, Seyed Sadegh Gharagheizi, Zahra Shahkolahi, Shaghayegh Sadeghi, Rezvaneh Vahedian Naderifar, Mahin Akbarizadeh, Majid Reza Soltaninejad, Simin
...
Published in
Biotechnology and bioengineering
Defending against antibiotic-resistant infections is similar to fighting a war with limited ammunition. As the new century unfolded, antibiotic resistance became a significant concern. In spite of the fact that phage treatment has been used as an effective means of fighting infections for more than a century, researchers have had to overcome many c...
Daffern, Nicolas Francino-Urdaniz, Irene M Baumer, Zachary T Whitehead, Timothy A
Published in
Biotechnology and bioengineering
Protocols for the construction of large, deeply mutagenized protein encoding libraries via Golden Gate assembly of synthetic DNA cassettes employ disparate, system-specific methodology. Here we present a standardized Golden Gate method for building user-defined libraries. We demonstrate that a 25 μL reaction, using 40 fmol of input DNA, can generat...
Chowdhury, Srijita Sarkar, Nandini
Published in
Biotechnology and bioengineering
Amyloid is defined as a fibrous quaternary structure formed by assembling protein or peptide monomers into intermolecularly hydrogen linked β-sheets. There is a prevalent issue with protein aggregation and the buildup of amyloid molecules, which results in human neurological illnesses including Alzheimer's and Parkinson's. But it is now evident tha...
Williams, Tyrell J Daboin, Claudia Kim, Paul Guo, Fengguang de Figueiredo, Paul Alge, Daniel L
Published in
Biotechnology and bioengineering
Methods for culturing oxygen-sensitive cells and organisms under anaerobic conditions are vital to biotechnology research. Here, we report a biomaterial-based platform for anaerobic culture that consists of glucose oxidase (GOX) functionalized alginate microparticles (ALG-GOX), which are designed to deplete dissolved [O2 ] through enzymatic activit...
Anderson, Hanna Hersh, David S Khan, Yusuf
Published in
Biotechnology and bioengineering
Pediatric patients suffering traumatic brain injuries may require a decompressive craniectomy to accommodate brain swelling by removing a portion of the skull. Once the brain swelling subsides, the preserved calvarial bone flap is ideally replaced as an autograft during a cranioplasty to restore protection of the brain, as it can reintegrate and gr...
