Li, Zonglin Sun, Chuanqi Lou, Longwei Li, Zhimin
Published in
Biotechnology and bioengineering
The pyrimidine metabolic pathway is tightly regulated in microorganisms, allowing limited success in metabolic engineering for the production of pathway-related substances. Here, we constructed a four-enzyme coupled system for the in vitro production of uridine triphosphate (UTP). The enzymes used include nucleoside kinase, uridylate kinase, nucleo...
MacDonald, Michael A Barry, Craig Groves, Teddy Martínez, Verónica S Gray, Peter P Baker, Kym Shave, Evan Mahler, Stephen Munro, Trent Marcellin, Esteban
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Published in
Biotechnology and bioengineering
Chinese hamster ovary (CHO) cells are the primary platform for the production of biopharmaceuticals. To increase yields, many CHO cell lines have been genetically engineered to resist cell death. However, the kinetics that governs cell fate in bioreactors are confounded by many variables associated with batch processes. Here, we used CRISPR-Cas9 to...
Lin, Bobo Huang, Gangliang
Published in
Biotechnology and bioengineering
White jellyfungus is one of the most popular nutritional supplements. The polysaccharide (WJP) is an important active component of White jellyfungus, it not only has a variety of biological activities but also is nontoxic to humans. So, many scholars have carried out different research on WJP. However, the lack of a detailed summary of WJP limits t...
Li, Xiaobo Ke, Xiang Qiao, Lijia Sui, Yufei Chu, Ju
Published in
Biotechnology and bioengineering
Omics approaches have been applied to understand the boosted productivity of natural products by industrial high-producing microorganisms. Here, with the updated genome sequence and transcriptomic profiles derived from high-throughput sequencing, we exploited comparative omics analysis to further enhance the biosynthesis of erythromycin in an indus...
McFarland, Kevin S Zhu, Jie Sinharoy, Pritam Betenbaugh, Michael J Handlogten, Michael W
Published in
Biotechnology and bioengineering
The production of biologics that treat complex diseases, such as cancer, autoimmune, and infectious disease, requires careful monitoring and control of cell cultures. While bioprocess optimizations have dramatically improved production yields, a lack of analytical tools has made it challenging to identify accompanying intracellular improvements. In...
Liu, Qi Lu, Minping Jin, Ci Hou, Weiliang Zhao, Liao Bao, Jie
Published in
Biotechnology and bioengineering
Microbial lipid production from lignocellulose biomass provides an essential option for sustainable and carbon-neutral supply of future aviation fuels, biodiesel, as well as various food and nutrition products. Oleaginous yeast is the major microbial cell factory but its lipid-producing performance is far below the requirements of industrial applic...
Shankar, Uttara Lenka, Sangram K Ackland, M Leigh Callahan, Damien L
Published in
Biotechnology and bioengineering
Microalgae are important renewable feedstock to produce biodiesel and high-value chemicals. Different wavelengths of light influence the growth and metabolic activities of algae. Recent research has identified the light-sensing proteins called photoreceptors that respond to blue or red light. Structural elucidations of algal photoreceptors have gai...
Heredia, Vladimir Marchal, Luc Gonçalves, Olivier Pruvost, Jeremy
Published in
Biotechnology and bioengineering
Nitrogen limitation and changing solar conditions are both known to affect triacylglycerol (TAG) production in microalgae. This study investigates the optimization of TAG production with a continuous nitrogen-limited culture of Nannochloropsis gaditana in simulated day-night cycles (DNc). The effect of DNc was first investigated in nitrogen-deprive...
Shin, Nari Kim, Youngtaek Ko, Jihoon Choi, Soon Won Hyung, Sujin Lee, Seung-Eun Park, Seunghyuk Song, Jiyoung Jeon, Noo Li Kang, Kyung-Sun
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Published in
Biotechnology and bioengineering
In vitro platforms for studying the human brain have been developed, and brain organoids derived from stem cells have been studied. However, current organoid models lack three-dimensional (3D) vascular networks, limiting organoid proliferation, differentiation, and apoptosis. In this study, we created a 3D model of vascularized spheroid cells using...
Arnling Bååth, Jenny Novy, Vera Carneiro, Leonor V Guebitz, Georg M Olsson, Lisbeth Westh, Peter Ribitsch, Doris
Published in
Biotechnology and bioengineering
Cutinases can play a significant role in a biotechnology-based circular economy. However, relatively little is known about the structure-function relationship of these enzymes, knowledge that is vital to advance optimized, engineered enzyme candidates. Here, two almost identical cutinases from Thermobifida cellulosilytica DSM44535 (Thc_Cut1 and Thc...
