Chemmalil, Letha Prabhakar, Tanushree Kuang, June West, Jay Tan, Zhijun Ehamparanathan, Vivekh Song, Yuanli Xu, Jianlin Ding, Julia Li, Zhengjian
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Published in
Biotechnology and bioengineering
Process analytical technology (PAT) has been defined by the Food and Drug Administration as a system for designing, analyzing, and controlling manufacturing through timely measurements to ensure final product quality. Based on quality-by-design (QbD) principles, real-time or near-real-time data monitoring is essential for timely control of critical...
Zhang, Liang Ren, Shichao Liu, Xiaofei Liu, Xiaochen Guo, Fang Sun, Wentao Feng, Xudong Li, Chun
Published in
Biotechnology and bioengineering
Pentacyclic triterpenoids have wide applications in the pharmaceutical industry. The precise glucosylation at C-3 OH of pentacyclic triterpenoids mediated by uridine 5'-diphospho-glucosyltransferase (UDP-glucosyltransferase [UGT]) is an important way to produce valuable derivatives with various improved functions. However, most reported UGTs suffer...
Roush, David Asthagiri, Dilip Babi, Deenesh K Benner, Steve Bilodeau, Camille Carta, Giorgio Ernst, Philipp Fedesco, Mark Fitzgibbon, Sean Flamm, Matthew
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Published in
Biotechnology and bioengineering
The Third Modeling Workshop focusing on bioprocess modeling was held in Kenilworth, NJ in May 2019. A summary of these Workshop proceedings is captured in this manuscript. Modeling is an active area of research within the biotechnology community, and there is a critical need to assess the current state and opportunities for continued investment to ...
Balasubramanian, Sowmiya Chandrasekran, Priyadharshini Yesudhas, Anitha J R Ganapathyraman, Padmapriya Eiteman, Mark A Subramanian, Ramalingam
Published in
Biotechnology and bioengineering
To maximize the productivity of engineered metabolic pathway, in silico model is an established means to provide features of enzyme reaction dynamics. In our previous study, Escherichia coli engineered with acrylate pathway yielded low propionic acid titer. To understand the bottleneck behind this low productivity, a kinetic model was developed tha...
Yang, Haijian Hu, Chunxiang
Published in
Biotechnology and bioengineering
Microalgae can accumulate a large fraction of reduced carbon as lipids under NaCl stress. This study investigated the mechanism of carbon allocation and reduction and triacylglycerol (TAG) accumulation in microalgae under NaCl-induced stress. Micractinium sp. XJ-2 was exposed to NaCl stress and cells were subjected to physiological, biochemical, an...
Dash, Biraja C Duan, Kaiti Xing, Hao Kyriakides, Themis R Hsia, Henry C
Published in
Biotechnology and bioengineering
Human-induced pluripotent stem cell-derived vascular smooth muscle cells (hiPSC-VSMCs) with proangiogenic properties have huge therapeutic potential. While hiPSC-VSMCs have already been utilized for wound healing using a biomimetic collagen scaffold, an in situ forming hydrogel mimicking the native environment of skin offers the promise of hiPSC-VS...
Liu, Tengfei Li, Jun Qiu, Lequan Zhang, Fuming Linhardt, Robert J Zhong, Weihong
Published in
Biotechnology and bioengineering
Dibutyl phthalate (DBP) is an environmental pollutant that can threaten human health. The strain Arthrobacter sp. ZJUTW, isolated from the sludge of a river of Hangzhou, can efficiently degrade DBP. Its genomic and transcriptomic differences when cultivated with DBP compared with glucose revealed specific DBP metabolic pathways in the ZJUTW strain....
Straub, Christopher T Bing, Ryan G Otten, Jonathan K Keller, Lisa M Zeldes, Benjamin M Adams, Michael W W Kelly, Robert M
Published in
Biotechnology and bioengineering
The production of volatile industrial chemicals utilizing metabolically engineered extreme thermophiles offers the potential for processes with simultaneous fermentation and product separation. An excellent target chemical for such a process is acetone (Tb = 56°C), ideally produced from lignocellulosic biomass. Caldicellulosiruptor bescii (Topt 78°...
Youngblood, Richard Flesher, Carmen G Delproposto, Jennifer Baker, Nicki A Neeley, Christopher K Li, Fanghua Lumeng, Carey N Shea, Lonnie D O'Rourke, Robert W
Published in
Biotechnology and bioengineering
Dysfunctional adipose tissue plays a central role in the pathogenesis of the obesity-related metabolic disease, including type 2 diabetes. Targeting adipose tissue using biopolymer implants is a novel therapeutic approach for metabolic disease. We transplanted porous poly(lactide-co-glycolide) (PLG) implants coated with human interleukin-4 (hIL-4)-...
Jia, Xiaoqiang Li, Ying Xu, Tao Wu, Kang
Published in
Biotechnology and bioengineering
Cell surface display of heavy metal-binding proteins has been used to enhance the adsorption capacity of heavy metals and the engineered microbial cells can be potentially used for the bioremediation of heavy metals. In this study, the proteins PbrR, PbrR691, and PbrD from the Cupriavidus metallidurans strain CH34 were displayed on the extracellula...
