Prem, Eva Maria Markt, Rudolf Wunderer, Mathias Wagner, Andreas Otto
Published in
Biotechnology and bioengineering
An efficient biogas production out of organic (waste) materials is important to contribute to a carbon-neutral future. In this study, thermophilic press water (PW) coming from an organic fraction of the municipal solid waste digester was further digested in a thermo- and mesophilic posttreatment approach using two semicontinuous 14 L digesters. The...
Pogodaev, Aleksandr Hernández Rodríguez, Tanja Li, Mengyao García Münzer, David Gerardo
Published in
Biotechnology and bioengineering
Improving bioprocess efficiency is important to reduce the current costs of biologics on the market, bring them faster to the market, and to improve the environmental footprint. The process intensification efforts were historically focused on the main stage, while intensification of pre-stages has started to gain attention only in the past decade. ...
Belliveau, Jessica Thompson, Will Papoutsakis, Eleftherios T
Published in
Biotechnology and bioengineering
Chinese hamster ovary (CHO) cells release and exchange large quantities of extracellular vesicles (EVs). EVs are highly enriched in microRNAs (miRs, or miRNAs), which are responsible for most of their biological effects. We have recently shown that the miR content of CHO EVs varies significantly under culture stress conditions. Here, we provide a n...
Besada-Lombana, Pamela B Chen, Wilfred Da Silva, Nancy A
Published in
Biotechnology and bioengineering
The efficient hydrolysis of lignocellulosic biomass into fermentable sugars is key for viable economic production of biofuels and biorenewable chemicals from second-generation feedstocks. Consolidated bioprocessing (CBP) combines lignocellulose saccharification and chemical production in a single step. To avoid wasting valuable resources during CBP...
Oh, Young Hoon Mendola, Kerri M Choe, Leila H Min, Lie Lavoie, Ashton R Sripada, Sobhana A Williams, Taufika Islam Lee, Kelvin H Yigzaw, Yinges Seay, Alexander
...
Published in
Biotechnology and bioengineering
Host-cell proteins (HCPs) are the foremost class of process-related impurities to be controlled and removed in downstream processing steps in monoclonal antibody (mAb) manufacturing. However, some HCPs may evade clearance in multiple purification steps and reach the final drug product, potentially threatening drug stability and patient safety. This...
Cao, Thu M Chen, Dayue Barnard, Gavin C Shen, Amy
Published in
Biotechnology and bioengineering
The gene therapy field has advanced in recent years with five recombinant adeno-associated virus (rAAV) based products winning Food and Drug Administration (FDA) approval. As the number of therapeutic applications and overall production demands for rAAV increase, it is valuable to evaluate rAAV production in different production cells. Chinese hams...
Farcet, Maria R Modrof, Jens Antoine, Gerhard Klausen, Cecilie Kerschbaum, Astrid Kopp, Martina Dehghani, Houman Kreil, Thomas R
Published in
Biotechnology and bioengineering
Minute virus of mice (MMV) has contaminated biotechnological processes in the past and specific MMV testing is therefore recommended, if the production cell line is known to be permissive for this virus. Testing is widely done using cell-culture-based adventitious virus assays, yet MMV strains may differ in their in vitro cell tropism. Here, we inv...
Wang, Yongfa Pan, Hao Wang, Fuhao Shen, Chen
Published in
Biotechnology and bioengineering
Cytochrome P450s (CYPs) are heme-thiolated enzymes that catalyze the oxidation of C-H bonds in a regio- and stereo-selective manner. CYPs are widely present in the biological world. With the completion of more biological genome sequencing, the number and types of P450 enzymes have increased rapidly. P450 in microorganisms is easy to clone and expre...
Fekete-Kertész, Ildikó Pous, Narcís Feigl, Viktória Márton, Rita Berkl, Zsófia Ceballos-Escalera, Alba Balaguer, Maria D Puig, Sebastià Molnár, Mónika
Published in
Biotechnology and bioengineering
The performance of combined reduction of nitrate (NO3 - ) to dinitrogen gas (N2 ) and oxidation of arsenite (As[III]) to arsenate (As[V]) by a bioelectrochemical system was assessed, supported by ecotoxicity characterization. For the comprehensive toxicity characterization of the untreated model groundwater and the treated reactor effluents, a prob...
Weiß, Linus Schmieder-Todtenhaupt, Valerie Haemmerling, Frank Lakatos, Daniel Schulz, Patrick Fischer, Simon
Published in
Biotechnology and bioengineering
A large number of companies observe polysorbate (PS) degradation and associated (sub-)visible particle formation in biological drug formulations, which compromise the stability of the drug product, ultimately posing a risk toward delivering innovative medicines to patients. The main culprits of PS degradation are hydrolytic host cell proteins (HCPs...
