Petrášek, Zdeneˇk Eibinger, Manuel Nidetzky, Bernd
Published in
Biotechnology and bioengineering
The hydrolysis of cellulose by processive cellulases, such as exocellulase TrCel7A from Trichoderma reesei, is typically characterized by an initial burst of high activity followed by a slowdown, often leading to incomplete hydrolysis of the substrate. The origins of these limitations to cellulose hydrolysis are not yet fully understood. Here, we p...
Dopp, Jared Lynn Rothstein, Samuel Michael Mansell, Thomas Joseph Reuel, Nigel Forest
Published in
Biotechnology and bioengineering
In this study, we present a minimal template design and accompanying methods to produce assayable quantities of custom sequence proteins within 24 hr from receipt of inexpensive gene fragments from a DNA synthesis vendor. This is done without the conventional steps of plasmid cloning or cell-based amplification and expression. Instead the linear te...
Bolivar, Juan M Mannsberger, Alexander Thomsen, Malene S Tekautz, Günter Nidetzky, Bernd
Published in
Biotechnology and bioengineering
Oxidative O2 -dependent biotransformations are promising for chemical synthesis, but their development to an efficiency required in fine chemical manufacturing has proven difficult. General problem for process engineering of these systems is that thermodynamic and kinetic limitations on supplying O2 to the enzymatic reaction combine to create a com...
Emami, Parinaz Motevalian, Seyed Pouria Pepin, Erin Zydney, Andrew L
Published in
Biotechnology and bioengineering
Conjugated vaccines prepared from the capsular polysaccharide of Streptococcus pneumoniae can provide immunization against invasive pneumococcal disease, meningitis, and otitis media. One of the critical steps in the production of these vaccines is the removal of free (unreacted) polysaccharides from the protein-polysaccharide conjugate. Experiment...
Fernandez-Cerezo, Lara Rayat, Andrea C M E Chatel, Alex Pollard, Jennifer M Lye, Gary J Hoare, Michael
Published in
Biotechnology and bioengineering
The availability of material for experimental studies is a key constraint in the development of full-scale bioprocesses. This is especially true for the later stages in a bioprocess sequence such as purification and formulation, where the product is at a relatively high concentration and traditional scale-down models can require significant volumes...
Hemmerich, Johannes Moch, Matthias Jurischka, Sarah Wiechert, Wolfgang Freudl, Roland Oldiges, Marco
Published in
Biotechnology and bioengineering
The impact of Sec signal peptides (SPs) from Bacillus subtilis in combination with isopropyl-β- d-1-thiogalactopyranoside concentration and feeding profile was investigated for heterologous protein secretion performance by Corynebacterium glutamicum using cutinase as model enzyme. Based on a comprehensive data set of about 150 bench-scale bioreacto...
Lee, Jinhee Son, Ahyun Kim, Paul Kwon, Soon Bin Yu, Ji Eun Han, Gyoonhee Seong, Baik L
Published in
Biotechnology and bioengineering
Transglutaminase (TGase) induces the cross-linking of proteins by catalyzing an acyl transfer reaction. TGase is a zymogen, activated by the removal of its pro-region. Because the pro-region is crucial for folding and inhibition of the TGase activity, the recombinant expression of the mature TGase (mTGase) without the pro-region, usually results in...
Chen, Ting-Hsuan Liu, Wen-Chun Lin, Chia-Ying Liu, Chia-Chyi Jan, Jia-Tsrong Spearman, Maureen Butler, Michael Wu, Suh-Chin
Published in
Biotechnology and bioengineering
Refocusing of B-cell responses can be achieved by preserving the overall fold of the antigen structure but selectively mutating the undesired antigenic sites with additional N-linked glycosylation motifs for glycan masking the vaccine antigen. We previously reported that glycan-masking recombinant H5 hemagglutinin (rH5HA) antigens on residues 83, 1...
Chen, Xiulai Li, Yang Tong, Tian Liu, Liming
Published in
Biotechnology and bioengineering
Fumarate is a naturally occurring organic acid that is an intermediate of the tricarboxylic acid (TCA) cycle and has numerous applications in food, pharmaceutical, and chemical industries. However, microbial fumarate production from renewable feedstock is limited by the intrinsic inefficiency of its synthetic pathway caused by week metabolites tran...
Mahameed, Mohamed Obiedat, Akram Beck, Gad Johnson, Jeffrey C Tirosh, Boaz
Published in
Biotechnology and bioengineering
Protein translation has emerged as a critical bottleneck for overall productivity of biological molecules. An augmentation of protein translation can be achieved by cell line engineering or by sophisticated vector design. However, for industrial process development purposes, identification of media additives that promote translation will be of grea...
