Ata, Özge Prielhofer, Roland Gasser, Brigitte Mattanovich, Diethard Çalık, Pınar
Published in
Biotechnology and bioengineering
The constitutive glyceraldehyde-3-phosphate dehydrogenase promoter (PGAP ), which is one of the benchmark promoters of Pichia pastoris, was analyzed in terms of putative transcription factor binding sites. We constructed a synthetic library with distinct regulatory properties through deletion and duplication of these putative transcription factor b...
Schinn, Song-Min Bradley, William Groesbeck, Ashtyn Wu, Jeffrey C Broadbent, Andrew Bundy, Bradley C
Published in
Biotechnology and bioengineering
The incorporation of unnatural amino acids (uAA) can introduce novel functional groups into proteins site-specifically, with important applications in basic sciences and protein engineering. However, uAA incorporation can impact protein expression and functional activity depending on its location within the protein-a process that is not yet complet...
Lee, Jeong H Reier, Jason Heffner, Kelley M Barton, Christopher Spencer, David Schmelzer, Albert E Venkat, Raghavan
Published in
Biotechnology and bioengineering
Coagulation factor II (prothrombin; FII) is the pre-proteolyzed precursor to thrombin in the coagulation cascade. It has 10 sites of gamma-carboxylation, which are required for its bioactivity, and is N-glycosylated at three of four putative sites. Production of recombinant human FII (rhFII) using a platform fed-batch process designed for monoclona...
Lonardo, Anthony Saxena Parekh, Babita Srivastava, Arvind
Published in
Biotechnology and bioengineering
Monoclonal antibody production processes control critical quality attributes (CQAs), which are the measures that provide proof of a product's identity and quality. Critical decisions rely on the accuracy and precision of these measures, as well as their appropriate statistical treatment. Many measures require special attention. For example, the cha...
Decker, Joseph T Hobson, Eric C Zhang, Yining Shin, Seungjin Thomas, Alexandra L Jeruss, Jacqueline S Arnold, Kelly B Shea, Lonnie D
Published in
Biotechnology and bioengineering
The development of resistance to targeted therapeutics is a challenging issue for the treatment of cancer. Cancers that have mutations in BRCA, a DNA repair protein, have been treated with poly(ADP-ribose) polymerase (PARP) inhibitors, which target a second DNA repair mechanism with the aim of inducing synthetic lethality. While these inhibitors ha...
Baek, Youngbin Singh, Nripen Arunkumar, Abhiram Borys, Michael Li, Zheng J Zydney, Andrew L
Published in
Biotechnology and bioengineering
Ultrafiltration (UF) is used for the final concentration and formulation of essentially all antibody-based therapeutics including both monoclonal antibodies (mAbs) and Fc-fusion proteins. The objective of this study was to quantitatively compare the filtrate flux behavior for two highly purified mAbs and an Fc-fusion protein under identical flow an...
Ee, Xueping Yan, Ying Hunter, Daniel A Schellhardt, Lauren Sakiyama-Elbert, Shelly E Mackinnon, Susan E Wood, Matthew D
Published in
Biotechnology and bioengineering
Providing temporally regulated glial cell line-derived neurotrophic factor (GDNF) to injured nerve can promote robust axon regeneration. However, it is poorly understood why providing highly elevated levels of GDNF to nerve can lead to axon entrapment in the zone containing elevated GDNF. This limited understanding represents an obstacle to the tra...
Odeleye, Akinlolu Oyekunle Oluseun Castillo-Avila, Sara Boon, Mathew Martin, Haydn Coopman, Karen
Published in
Biotechnology and bioengineering
The emergence of medicinal indications for stem cell therapies has seen a need to develop the manufacturing capacity for adherent cells such as mesenchymal stem cells (MSCs). One such development is in the use of microcarriers, which facilitate enhanced cell densities for adherent stem cell cultures when compared with 2D culture platforms. Given th...
Zeng, Wendy R Beh, Siew-Joo Bryson-Richardson, Robert J Doran, Pauline M
Published in
Biotechnology and bioengineering
The hearts of adult zebrafish (Danio rerio) are capable of complete regeneration in vivo even after major injury, making this species of particular interest for understanding the growth and differentiation processes required for cardiac tissue engineering. To date, little research has been carried out on in vitro culture of adult zebrafish cardiac ...
Koepff, Joachim Keller, Matthias Tsolis, Konstantinos C Busche, Tobias Rückert, Christian Hamed, Mohamed B Anné, Jozef Kalinowski, Joern Wiechert, Wolfgang Economou, Anastassios
...
Published in
Biotechnology and bioengineering
Filamentous organisms of the genus Streptomyces play an important role in industrial production processes, due to their extensive secondary metabolism variability, as well as their ability to secrete efficiently large amounts of (heterologous) proteins. While genetic engineering tools are available to rapidly build up large strain libraries, the su...
