Tescione, Lia Lambropoulos, James Paranandi, Madhava Ram Makagiansar, Helena Ryll, Thomas
Published in
Biotechnology and bioengineering
A bench scale cell culture model representative of manufacturing scale (2,000 L) was developed based on oxygen mass transfer principles, for a CHO-based process producing a recombinant human protein. Cell culture performance differences across scales are characterized most often by sub-optimal performance in manufacturing scale bioreactors. By cont...
Luterbacher, Jeremy S Moran-Mirabal, Jose M Burkholder, Eric W Walker, Larry P
Published in
Biotechnology and bioengineering
Enzymatic hydrolysis is one of the critical steps in depolymerizing lignocellulosic biomass into fermentable sugars for further upgrading into fuels and/or chemicals. However, many studies still rely on empirical trends to optimize enzymatic reactions. An improved understanding of enzymatic hydrolysis could allow research efforts to follow a ration...
Glebes, Tirzah Y Sandoval, Nicholas R Gillis, Jacob H Gill, Ryan T
Published in
Biotechnology and bioengineering
Engineering both feedstock and product tolerance is important for transitioning towards next-generation biofuels derived from renewable sources. Tolerance to chemical inhibitors typically results in complex phenotypes, for which multiple genetic changes must often be made to confer tolerance. Here, we performed a genome-wide search for furfural-tol...
Tamura, Akio Fukutani, Yosuke Takami, Taku Fujii, Motoko Nakaguchi, Yuki Murakami, Yoshihiko Noguchi, Keiichi Yohda, Masafumi Odaka, Masafumi
Published in
Biotechnology and bioengineering
The encapsulin nanocompartment from Rhodococcus erythropolis N771 (Reencapsulin) was expressed and purified in wild-type and C-terminally His-tagged forms. Negative-stained transmission electron microscopy, field-flow fractionation combined with multi-angle light scattering and dynamic light scattering analyses showed that 60 Reencapsulin monomers ...
Chen, Xiulai Wu, Jing Song, Wei Zhang, Limei Wang, Hongjiang Liu, Liming
Published in
Biotechnology and bioengineering
A multi-vitamin auxotrophic Torulopsis glabrata strain, a pyruvate producer, was further engineered to produce fumaric acid. Using the genome-scale metabolic model iNX804 of T. glabrata, four fumaric acid biosynthetic pathways, involving the four cytosolic enzymes, argininosuccinate lyase (ASL), adenylosuccinate lyase (ADSL), fumarylacetoacetase (F...
Du, Zhimei Treiber, David McCarter, John D Fomina-Yadlin, Dina Saleem, Ramsey A McCoy, Rebecca E Zhang, Yuling Tharmalingam, Tharmala Leith, Matthew Follstad, Brian D
...
Published in
Biotechnology and bioengineering
The continued need to improve therapeutic recombinant protein productivity has led to ongoing assessment of appropriate strategies in the biopharmaceutical industry to establish robust processes with optimized critical variables, that is, viable cell density (VCD) and specific productivity (product per cell, qP). Even though high VCD is a positive ...
Pásztor, András Kallio, Pauli Malatinszky, Dávid Akhtar, M Kalim Jones, Patrik R
Published in
Biotechnology and bioengineering
Several synthetic metabolic pathways for butanol synthesis have been reported in Escherichia coli by modification of the native CoA-dependent pathway from selected Clostridium species. These pathways are all dependent on the O2 -sensitive AdhE2 enzyme from Clostridium acetobutylicum that catalyzes the sequential reduction of both butyryl-CoA and bu...
Park, Seongjun Chung, Jinwook Rittmann, Bruce E Bae, Wookeun
Published in
Biotechnology and bioengineering
To achieve nitrite accumulation for shortcut biological nitrogen removal (SBNR) in a biofilm process, we explored the simultaneous effects of oxygen limitation and free ammonia (FA) and free nitrous acid (FNA) inhibition in the nitrifying biofilm. We used the multi-species nitrifying biofilm model (MSNBM) to identify conditions that should or shoul...
Chatterjee, Madhubanti Ge, Xudong Uplekar, Shaunak Kostov, Yordan Croucher, Leah Pilli, Manohar Rao, Govind
Published in
Biotechnology and bioengineering
Although online monitoring of dissolved oxygen (DO) and carbon dioxide (DCO2 ) is highly desirable in bioprocesses, small-scale bioreactors are usually not monitored due to the lack of suitable sensors. Traditional electrochemical sensors are usually not used because they are bulky and invasive. Disposable optical sensors are small and only partial...
Kagawa, Y Tahata, J Kishida, N Matsumoto, S Picioreanu, C van Loosdrecht, M C M Tsuneda, S
Published in
Biotechnology and bioengineering
We developed a model for nutrient removal in an aerobic granular sludge system. This model can quantitatively describe the start-up of the system by coupling a model for studying the population dynamics of the granules in the reactor (reactor-scale model) and a model for studying the microbial community structure in the granules (granule-scale mode...
