Wang, Zhengjun Zhang, Yulong Zhou, Qianqian Ma, Ping Wang, Xiaohui Zhan, Linsheng
Published in
Biotechnology Letters
ObjectiveTo monitor the inflammatory storage lesions of end-stage stored whole blood (SWB) using a noninvasive STAT3 signal pathway mouse model.ResultsIn this study, we present a hydrodynamic transfection-based STAT3-Luc mouse model in which hepatocyte STAT3 signal pathway activation can be monitored by measuring luciferase activity using a noninva...
Deb, Shalini S. Reshamwala, Shamlan M. S. Lali, Arvind M.
Published in
Biotechnology Letters
ObjectiveMetabolic engineering efforts are guided by identifying gene targets for overexpression and/or deletion. Isobutanol, a biofuel candidate, is biosynthesized using the valine biosynthesis pathway and enzymes of the Ehrlich pathway. Most reported studies for isobutanol production in Escherichia coli employ multicopy plasmids, an approach that...
Zhang, Yuqin Yang, Feng Ren, Hejun Liu, Jinliang Mu, Wenyang Wang, Yan
Published in
Biotechnology Letters
ObjectivesTo evaluate Pseudomonas monteilii strain PN1 for the removal efficiency of P-nitrophenol (PNP) in soils and its growth promotion of maize (Zea mays L.) seedlings under drought and saline–alkali stress.ResultsPN1 can survive in soils contaminated with PNP dosage between 90 and 155 mg/kg and considerably improved the removal PNP efficiency ...
Niapour, Ali Ghasemi Hamidabadi, Hatef Niapour, Nazila Mohammadi, Perham Sharifi Pasandi, Marzieh Malekzadeh, Vadoud
Published in
Biotechnology Letters
ObjectiveHuman dental pulp-derived stem cells (hDPSCs) are becoming an attractive source for cell-based neurorestorative therapies. As such, it is important to understand the molecular mechanisms that regulate the differentiation of hDPSCs toward the neuronal fate. Notch signaling plays key roles in neural stem/progenitor cells (NS/PCs) maintenance...
Mekala, Lakshmi Prasuna Mohammed, Mujahid Chintalapati, Sasikala Chintalapati, Venkata Ramana
Published in
Biotechnology Letters
ObjectiveTo explore the secondary metabolite biosynthetic potential of Rubrivivax benzoatilyticus JA2 using a new metabolite mining strategy.ResultsCombination of precursor-feeding and altered growth conditions were used to mine new biomolecules. Strain JA2 utilised l-phenylalanine as sole source of nitrogen and showed pigments production only unde...
Wang, Xiuwen Cheng, Yang Jin, Qingzhe Wei, Wei Wang, Xingguo
Published in
Biotechnology Letters
ObjectivesTo investigate the lipase-catalyzed synthesis of high purity sn-1/3 and sn-2 monoacylglycerols (1/3-MAG and 2-MAG) of different fatty acids (FAs).ResultsThe 1/3-MAGs of three FAs (16:0, 17:0, 16:1) were synthesized using lipase-catalyzed esterification of glycerol with FAs. The 2-MAGs were obtained from the ethanolysis of synthetic triacy...
Jiménez, N. Martínez, V. S. Gerdtzen, Z. P.
Published in
Biotechnology Letters
ObjectiveOver-express galactokinase (Galk1) in tissue plasminogen activator (tPA) producing CHO cells as a potential strategy to improve cell growth and product synthesis.ResultstPA producing CHO cells were transfected with the galactokinase (Galk1) gene. CHO-Galk1 cells showed a 39% increase of the specific growth rate in galactose. Moreover, clon...
Jiao, YuLiang Wu, Yong Chen, HongXu Wang, ShuJun Chen, Li Lv, MingSheng Fang, YaoWei Liu, Shu
Published in
Biotechnology Letters
ObjectiveTo determine the impact of the N-terminal nonessential domains on the enzymological properties of a pullulanase (BmP) from Bacillus megaterium strain P6.MethodsThe domains of BmP were identified by the conserved domain (CD) search online software. BmP was prepared by fermentation with the strain P6 and its N-terminal truncated form (BmNTP)...
Tucaliuc, Alexandra Blaga, Alexandra Cristina Galaction, Anca Irina Cascaval, Dan
Published in
Biotechnology Letters
Mupirocin is an antibiotic from monocarboxylic acid class used as antibacterial agent against methicillin-resistant Staphylococcus aureus (MRSA) and can be obtained as a mixture of four pseudomonic acids by Pseudomonas fluorescens biosynthesis. Nowadays improving antibiotics occupies an important place in the pharmaceutical industry as more and mor...
Yang, So-Jin Choi, Su-Jeong Park, Bo-Ram Kim, Young-Min
Published in
Biotechnology Letters
ObjectiveThe biochemical properties of a putative thermostable cycloisomaltooligosaccharide (CI) glucanotransferase gene from Thermoanaerobacter thermocopriae were determined using a recombinant protein (TtCITase) expressed in Escherichia coli and purified to a single protein.ResultsThe 171-kDa protein displayed maximum activity at pH 6.0, and enzy...
