Longo, Angela Russo, Pasquale Capozzi, Vittorio Spano, Giuseppe Fiocco, Daniela
Published in
Biotechnology Letters
Objective We investigated whether the knock out of small heat shock protein (sHSP) genes ( hsp1 , hsp2 and hsp3 ) impact on probiotic features of Lactiplantibacillus plantarum WCFS1, aiming to find specific microbial effectors involved in microbe-host interplay. Results The probiotic properties of L. plantarum WCFS1 wild type, hsp1 , hsp2 and hsp3 ...
Zieniuk, Bartłomiej Wołoszynowska, Małgorzata Białecka-Florjańczyk, Ewa Fabiszewska, Agata
Published in
Biotechnology Letters
Objective The aim of the study was to evaluate the possibility of using Y. lipolytica biomass as a whole-cell catalyst in the synthesis of lipophilic antioxidants, with the example of esterification of five phenolic acids with 1-butanol. Results Freeze-dried Y. lipolytica biomass was successfully applied as a biocatalyst in the synthesis of esters ...
Lee, J. Kim, T.-H. Oh, D.-K.
Published in
Biotechnology Letters
ObjectiveTo produce high concentrations of 13-hydroxy-14,15-epoxy-eicosatrienoic acid (14,15-hepoxilin B3, 14,15-HXB3) and 13,14,15-trihydroxy-eicosatrienoic acid (13,14,15-trioxilin B3, 13,14,15-TrXB3) from arachidonic acid (ARA) using microbial 15-lipoxygenase (15-LOX) without and with epoxide hydrolase (EH), respectively.ResultsThe products obta...
Jieming, Guo Liu, Chuan Yang, Yin Mo, Shanyi Yang, Xuesong Wang, Ju
Published in
Biotechnology letters
In interstitial fibrosis, alveolar epithelial type II (AE2) cells fail to repair damaged epithelium. However, whether this dysfunction is related to fibroblast growth factor (FGF) signal pathway and how it affects the fibrotic process remains unclear. In our study, the medium of the human foetal lung fibroblast cell line MRC-5 (Med) can induce epit...
Kardani, Kimia Bolhassani, Azam Agi, Elnaz Hashemi, Atieh
Published in
Biotechnology Letters
Objectives Enhancement of the potential ability of biomacromolecules to cross cell membranes is a critical step for development of effective therapeutic vaccine especially DNA vaccine against human immunodeficiency virus-1 (HIV-1) infection. The supercharged proteins were known as powerful weapons for delivery of different types of cargoes such as ...
Sabry, Dina Marzouk, Samar Zakaria, Reem Ibrahim, Heba A. Samir, Mai
Published in
Biotechnology Letters
AimThe aim of the current study was to evaluate the therapeutic and regenerative effects of MSCs derived exosomes in the treatment of type 1 DM and to compare its effects with MSCs themselves. The experiment was done on forty albino rats grouped as follows, group (1): Ten healthy rats, group (2): Ten induced type 1 DM rats, group (3): Ten induced t...
Liu, Xinsheng Qi, Linlin Lv, Jianliang Zhang, Zhongwang Zhou, Peng Ma, Zhongyuan Wang, Yonglu Zhang, Yongguang Pan, Li
Published in
Biotechnology Letters
Objective Development of an effective mucosal vaccine to induce specific immune responses against Foot-and-mouth disease virus (FMDV). Results For this purpose, the FMDV VP1 gene (SPVP1) was optimized and synthesized based on the codon bias of Lactococcus lactis ( L . lactis ), and then incorporated in the plasmid pNZ8148. L. lactis NZ9000 containi...
Grobe, Sascha Wszołek, Agata Brundiek, Henrike Fekete, Melinda Bornscheuer, Uwe T
Published in
Biotechnology letters
Regio- and stereoselective hydroxylation of lithocholic acid (LCA) using CYP107D1 (OleP), a cytochrome P450 monooxygenase from the oleandomycin synthesis pathway of Streptomyces antibioticus. Co-expression of CYP107D1 from S. antibioticus and the reductase/ferredoxin system PdR/PdX from Pseudomonas putida was performed in Escherichia coli whole cel...
Larroude, Macarena Trabelsi, Heykel Nicaud, Jean-Marc Rossignol, Tristan
Published in
Biotechnology letters
The construction and validation of a set of Yarrowia lipolytica CRISPR/Cas9 vectors containing six different markers that allows virtually any genetic background to be edited, including those of wild-type strains. Using the Golden Gate method, we assembled a set of six CRISPR/Cas9 vectors, each containing a different selection marker, to be used fo...
Park, Youngmin Lee, Sangmin Kang, Hyangju Park, Minhee Min, Kyungmin Kim, Nam Hyung Gu, Sungmin Kim, Jong Kook An, Dong-Jun Choe, SeEun
...
Published in
Biotechnology Letters
Classical swine fever (CSF) is one of the most important viral diseases of swine worldwide. Although live or attenuated virus vaccines have been used to control CSFV, it is difficult to distinguish vaccinated pigs from infected pigs; this leads to restrictions on import and export. Subunit vaccines based on the CSFV E2 glycoprotein have been develo...
