Kumari, Arti Gupta, Rani
Published in
Biotechnology Letters
A novel lipase gene TAlipC was isolated from Trichosporon asahii MSR54 and functionally expressed in Pichia pastoris. The protein was His-tagged and purified to homogeneity by affinity chromatography. Sequence comparison revealed a high homology with lipases from Cryptococcus sp. It had a GX type oxyanion hole and a GHSLG-type conserved penta-pepti...
Yu, Xianhong Zhai, Chao Zhong, Xing Tang, Wei Wang, Xiaojuan Yang, Hu Chen, Wanping Ma, Lixin
Published in
Biotechnology Letters
Carboxypeptidase Y is widely used in peptide sequencing and mass spectrometry. PRC1 coding for proteinase C from Saccharomyces cerevisiae was expressed in Pichia pastoris GS115 as procarboxypeptidase Y with a yield of ~605 mg/l in shake-flasks after 168 h induction with 1 % (v/v) methanol. This precursor of carboxypeptidase Y was cleaved by endogen...
Liu, Yan-Qiu Han, Xiao-Fei Liu, Tiegang Cheng, Meng-Chun Xiao, Hong-Bin
Published in
Biotechnology Letters
The activity of icarrin (a flavonoid from Herba epimedii) was investigated in the regulation of bone remodeling, a process coupled by osteoblast-mediated bone forming and osteoclast-mediated bone resorption. By directly co-culturing mouse bone marrow stromal cells and mouse preosteoclastic RAW264.7, and transwell co-culturing rat ovarian follicular...
Wang, Yu Liu, Jingjing Li, Qiu Wang, Yitao Wang, Chunming
Published in
Biotechnology Letters
Next-generation biomaterials are expected to possess both desirable mechanical features and unique biological functions. Recently, two plant-derived glucomannans (GMs)—Konjac glucomannan (KGM) and the polysaccharide of Bletilla striata (BSP)—have emerged as new sources for development of biomaterials. They have been fabricated into drug delivery ve...
Rafiqul, I. S. M. Sakinah, A. M. M. Zularisam, A. W.
Published in
Biotechnology Letters
Xylose reductase (XR) is an oxidoreductase having potential applications in the production of various specialty products, mainly xylitol. It is important to screen for compounds that can decrease XR activity and consequently can decrease xylitol production. We have identified the byproducts in the hemicellulosic hydrolysate that inhibit XR from Can...
Kanafi, Mohammad Mahboob Mamidi, Murali Krishna Sureshbabu, Shalini Kashipathi Shahani, Pradnya Bhawna, Chandravanshi Warrier, Sudha R. Bhonde, Ramesh
Published in
Biotechnology Letters
To explore a novel source for the derivation of islets, we examined the differentiation potential of human non-pancreatic cancer cell lines, HeLa (cervical carcinoma cell line) and MCF-7 (breast cancer cell line). These cells were subjected to a serum-free, three-step sequential differentiation protocol which gave two distinct cell populations: sin...
von der Haar, Daniela Stäbler, Andreas Wichmann, Rolf Schweiggert-Weisz, Ute
Published in
Biotechnology Letters
Different immobilized lipases were screened for their ability to esterify free fatty acids (FFA) with monoacylglycerol (MAG) as acyl-group acceptor. A lipase from Rhizomucor miehei (Lipozyme RMIM) was the most suitable for lipase-catalyzed de-acidification—a promising alternative to conventional neutralization. A reduction of the FFA content to 0.6...
Kremkow, Benjamin G. Lee, Kelvin H.
Published in
Biotechnology Letters
Over the last 10 years, 2nd and 3rd generation sequencing technologies have made the use of genomic sequencing within the animal cell culture community increasingly commonplace. Each technology’s defining characteristics are unique, including the cost, time, sequence read length, daily throughput, and occurrence of sequence errors. Given each seque...
He, Yu-Cai Tao, Zhi-Cheng Zhang, Dan-Ping Yang, Zhen-Xing Gao, Shan Ma, Cui-Luan
Published in
Biotechnology Letters
Liu, Jun Guo, Ting Wang, Dong Ying, Hanjie
Published in
Biotechnology Letters
A Clostridium beijerinckii mutant M13 was derived from C. beijerinckii NCIMB 8052 by atmospheric pressure glow discharge. C. beijerinckii M13 generated a maximum output power density of 79.2 mW m−2 and a maximum output voltage of 230 mV in a microbial fuel cell containing 1 g glucose l−1 as carbon source and 0.15 g methyl viologen l−1 as an electro...
