Sun, Da Sun, Zhanhu Jiang, Hongfa Vaidya, Amita M Xin, Rui Ayat, Nadia R Schilb, Andrew L Qiao, Peter L Han, Zheng Naderi, Amirreza
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Published in
Bioconjugate chemistry
CRISPR/Cas9 system is a promising approach for gene editing in gene therapy. Effective gene editing requires safe and efficient delivery of CRISPR/Cas9 system in target cells. Several new multifunctional pH-sensitive amino lipids were designed and synthesized with modification of the amino head groups for intracellular delivery of CRISPR/Cas9 syste...
Mukherjee, Ishita Ghosh, Anwesha Bhadury, Punyasloke De, Priyadarsi
Published in
Bioconjugate chemistry
Design of new drug material through modification of some well known antibiotics to combat pathogenic bacteria must include a complete understanding of matrix regulation, since the human body consists of majorly three types of matrices; solid, semisolid and liquid which has a tendency to regulate antibacterial efficacy along with bactericidal mechan...
Kawaguchi, Yoshimasa Ise, Shoko Azuma, Yusuke Takeuchi, Toshihide Kawano, Kenichi Le, Toan Khanh Ohkanda, Junko Futaki, Shiroh
Published in
Bioconjugate chemistry
Marked promotion of membrane permeation of a cell-penetrating peptide, octaarginine (R8), was attained by attachment to a single 2,2'-dipicolylamine moiety (DPA-R8) that forms 1:1 complexes with metal ions. Studies using giant unilamellar vesicles demonstrated that DPA targets phospholipids and enhances R8 binding to the membranes in the presence o...
Chambre, Laura Saw, Wen Shang Ekineker, Gülçin Kiew, Lik Voon Chong, Wu Yi Lee, Hong Boon Chung, Lip Yong Bretonniere, Yann Dumoulin, Fabienne Sanyal, Amitav
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International audience
Fink, Krzysztof Kobak, Kamil Kasztura, Monika Boratyński, Janusz Goszczyński, Tomasz M
Published in
Bioconjugate chemistry
Anionic boron clusters are man-made, inorganic compounds with potential applications in therapeutic peptides modification to improve their biological activity and pharmacokinetics, e.g., by enabling complexation with serum albumin. However, the conjugation of anionic boron clusters and peptides remains poorly understood. Here, we report a solid-sta...
Castillo, Rafael R Lozano, Daniel Vallet-Regí, María
Published in
Bioconjugate chemistry
Despite the claim that encapsulation of drugs improves the therapeutic profile of free drugs, there are still important limitations in drug delivery. With respect to cancer treatment, two promising implementations are combination therapy and targeted devices, which are aimed at increasing the drug effect either by achieving higher cell death rates ...
Güixens-Gallardo, Pedro Zawada, Zbigniew Matyašovský, Ján Dziuba, Dmytro Pohl, Radek Kraus, Tomáš Hocek, Michal
Published in
Bioconjugate chemistry
Synthesis of cytosine, uracil, and 7-deazaadenine 2'-deoxyribonucleosides and triphosphates (dNTPs) bearing hexamethylated phenyl-bodipy fluorophore attached at position 5 of pyrimidines or at position 7 of 7-deazapurine was developed. All the title labeled nucleosides and dNTPs displayed bright green fluorescence with very high quantum yields. The...
Vallade, Maëlle Jewginski, Michal Fischer, Lucile Buratto, Jérémie Bathany, Katell Schmitter, Jean-Marie Stupfel, Marine Godde, Frédéric Mackereth, Cameron D Huc, Ivan
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Published in
Bioconjugate chemistry
Helically folded aromatic foldamers may constitute suitable candidates for the ab initio design of ligands for protein surfaces. As preliminary steps toward the exploration of this hypothesis, a tethering approach was developed to detect interactions between a protein and a foldamer by confining the former at the surface of the latter. Cysteine mut...
Sugimoto, Shunsuke Iwasaki, Yasuhiko
Published in
Bioconjugate chemistry
Antigen-presenting cells play a dominant role in cancer immunotherapy. Tumor cells, however, can still resort to several mechanisms of immune evasion that ultimately lead to the development of tumor tissues. In the current study, we performed surface modification of live macrophages with nucleic acid aptamers with the aim to enhance their affinity ...
Wang, Jianxin Thomas, Mini Lin, Peng Cheng, Ji-Xin Matei, Daniela E Wei, Alexander
Published in
Bioconjugate chemistry
We present a robust method for loading small interfering RNA (siRNA) duplexes onto the surfaces of gold nanorods (GNRs) at high density, using near-infrared laser irradiation to trigger their intracellular release with subsequent knockdown activity. Citrate-stabilized GNRs were first coated with oleylsulfobetaine, a zwitterionic amphiphile with low...
