Kolínská, J Kraml, J Zákostelecká, M Kadlecová, L
Published in
Biochemistry international
To investigate the regulatory role of hydrocortisone in the postnatal decline of sialylation of intestinal brush-border hydrolases, we applied actinomycin D, the well-known inhibitor of transcription, simultaneously with the hormone to 9-day-old rats. On day 12 actinomycin D inhibited hydrocortisone-induced changes in the profile of enzyme activiti...
Okada, H Suzuki, J
Published in
Biochemistry international
The rate of ATP formation from ADP by adenylate kinase is known to be easily followed by determination of the increase in fluorescence due to the NADPH that is formed by combined reactions of hexokinase and gluc-6-p dehydrogenase. We found that the rate of CTP formation from CDP also can be followed similarly by use of more units of hexokinase and ...
Grant, A J
Published in
Biochemistry international
Modifications to the commonly employed lysine Sepharose affinity chromatography method for the purification of plasminogen from human plasma, give a preparation of native, Glu-plasminogen which is free of plasmin contamination.
Schmidt, M Löffler, B M Kunze, H
Published in
Biochemistry international
When rat hepatocytes were cultured in the presence of various specific protease inhibitors, lysosomal acid phospholipase A1 activity decreased progressively. Exposure of the cultured cells to 0.1 micrograms/ml of pepstatin, E 64, leupeptin or chymostatin also reduced the catalytic activities of several lysosomal marker enzymes. Irrespective of the ...
Luque, P Paredes, S Segura, J A Núñez de Castro, I Medina, M A
Published in
Biochemistry international
Interactions between glucose and glutamine metabolisms have been studied in a line of Ehrlich ascites tumour cells and the results obtained are compared with those recently published for ascites Lettré cells. In contrast with Lettré cells, in the less malignant line used in this work, glucose did not modify glutaminolytic flux and glutamine changed...
Hosokawa, Y Suzuki, H Toda, H Nishikimi, M Ozawa, T
Published in
Biochemistry international
Core protein II of mitochondrial cytochrome bc1 complex is the second largest nuclear-encoded subunit. We have isolated two overlapping cDNA clones encoding the precursor to this protein by screening a rat liver cDNA library, and determined the nucleotide sequence of the cDNAs. Based on the deduced amino acid sequence of the corresponding human pre...
Pugazhenthi, S Mantha, S V Khandelwal, R L
Published in
Biochemistry international
Effects of streptozotocin-induced diabetes and administration of the insulinmimetic agent, vanadate in rats on the liver protein kinase C-induced phosphorylation of exogenous C (Histone III-S) and endogenous substrates were investigated. Diabetes caused a significant fall (40-60%) in liver cytosolic protein C activity measured using both types of s...
Bobkova, E V Gedrovitch, A V Ankilova, V N Lavrik, O I Baratova, L A Shishkov, A V
Published in
Biochemistry international
A comparative study of thermostability and aminoacid composition of the phenylalanyl-tRNA synthetases from E. coli and Thermus thermophilus HB8 has been carried out. Compared with the mesophilic enzyme, a considerable increase of Pro, Leu, Phe, Arg and decrease of Asx, Ile, Ser, Thr and Lys content have been revealed in the thermophilic protein. Us...
Barua, M Haldar, S Majumder, G C
Published in
Biochemistry international
Goat cauda-epididymal intact sperm ecto [32P] proteins phosphorylated in presence of exogenous [gamma-32P]ATP by an endogenous ecto-cyclic AMP-independent protein kinase (CIK), have been found to lose 32P when the labelled cells are incubated at 37 degrees C in a modified Ringer's solution. Analysis of the 32P-labelled products of the turnover of t...
Murata, T Hibasami, H Maekawa, S Tagawa, T Nakashima, K
Published in
Biochemistry international
Effects of cisplatin on mitochondria in human malignant melanoma from gingiva were investigated. Cisplatin bound about 50 times more to mitochondrial DNA than to chromosomal DNA. NADH-ubiquinone reductase was decreased 48 h after the administration to about 40% of the initial level, and cellular ATP level was also depressed at that time. A good cor...
