Edmondson, Carina Zhou, Qi Liu, Xuan
Published in
BMC Biotechnology
BackgroundThe use of CRISPR/Cas9 technologies in generating single-base pair knock-in mutations has recently exploded in the number of methods available. However, with the growing expansion of new technologies, it can be difficult to determine the best method for genome editing.ResultsIn this study, we evaluated a number of CRISPR/Cas9 approaches f...
Li, Qin Xia, Ying Zhao, Ting Gong, Yuanyuan Fang, Shangling Chen, Maobin
Published in
BMC Biotechnology
Background4-vinylphenols produced by phenolic acid degradation catalyzed by phenolic acid decarboxylase can be used in food additives as well as flavor and fragrance industry. Improving the catalytic characters of phenolic acid decarboxylase is of great significance to enhance its practical application.ResultsA phenolic acid decarboxylase (P-WT) wa...
Bryan, Laura Henry, Michael Kelly, Ronan M. Frye, Christopher C. Osborne, Matthew D. Clynes, Martin Meleady, Paula
Published in
BMC Biotechnology
BackgroundThe ability to achieve high peak viable cell density earlier in CHO cell culture and maintain an extended cell viability throughout the production process is highly desirable to increase recombinant protein yields, reduce host cell impurities for downstream processing and reduce the cost of goods. In this study we implemented label-free L...
Wu, Ting Wang, Yinghua Xiao, Tianxiong Ai, Yirui Li, Jinsong Zeng, Yi Arial Yu, Qing Cissy
Published in
BMC Biotechnology
BackgroundProtein C receptor (Procr) has recently been shown to mark resident adult stem cells in the mammary gland, vascular system, and pancreatic islets. More so, high Procr expression was also detected and used as indicator for subsets of triple-negative breast cancers (TNBCs). Previous study has revealed Procr as a target of Wnt/β-catenin sign...
Gómez-Melero, Sara García-Maceira, Fé Isabel García-Maceira, Tania Luna-Guerrero, Verónica Montero-Peñalvo, Gracia Túnez-Fiñana, Isaac Paz-Rojas, Elier
Published in
BMC Biotechnology
BackgroundCCR6 chemokine receptor is an important target in inflammatory diseases. Th17 cells express CCR6 and a number of inflammatory cytokines, including IL-17 and IL-22, which are involved in the propagation of inflammatory immune responses. CCR6 antagonist would be a potential treatment for inflammatory diseases such as psoriasis or rheumatoid...
Aoki, Jinichi Sasaki, Daisaku Asayama, Munehiko
Published in
BMC Biotechnology
BackgroundMost commercial phycocyanins are extracted from a filamentous cyanobacterium, Arthrospira (Spirulina) platensis. Owing to the expenses of culture and complexities of the physical and chemical methods of phycocyanin purification, a more effective and simple method is required.ResultsWe developed a new method for efficiently recovering the ...
Li, Jing Yu, Jingjing Xue, Wenxian Huang, Huili Yan, Longjun Sang, Fan An, Shuangshuang Zhang, Jing Wang, Mingli Zhang, Jun
...
Published in
BMC Biotechnology
BackgroundHSPB5 is an ATP-independent molecular chaperone that is induced by heat shock or other proteotoxic stresses. HSPB5 is cytoprotective against stress both intracellularly and extracellularly. It acts as a potential therapeutic candidate in ischemia-reperfusion and neurodegenerative diseases.ResultsIn this paper, we constructed a recombinant...
Yahaya, M. A. F. Bakar, A. R. Abu Stanslas, J. Nordin, N. Zainol, M. Mehat, M. Z.
Published in
BMC Biotechnology
BackgroundNeuroinflammation has been identified to be the key player in most neurodegenerative diseases. If neuroinflammation is left to be unresolved, chronic neuroinflammation will be establish. Such situation is due to the overly-activated microglia which have the tendency to secrete an abundance amount of pro-inflammatory cytokines into the neu...
Ardila-Leal, Leidy D. Monterey-Gutiérrez, Pedro A. Poutou-Piñales, Raúl A. Quevedo-Hidalgo, Balkys E. Galindo, Johan F. Pedroza-Rodríguez, Aura M.
Published in
BMC Biotechnology
BackgroundLaccases (EC 1.10.3.2) are multi-copper oxidoreductases with great biotechnological importance due to their high oxidative potential and utility for removing synthetic dyes, oxidizing phenolic compounds, and degrading pesticides, among others.MethodsA real-time stability study (RTS) was conducted for a year, by using enzyme concentrates f...
Alyethodi, R. R. Singh, U. Kumar, S. Alex, R. Sengar, G. S. Raja, T. V. Deb, R. Prakash, B.
Published in
BMC Biotechnology
BackgroundDNA testing in the cattle industry undergoes multiple hurdles. Successful genotyping involves the transportation of samples from the field to the laboratory in a chilled environment followed by DNA extraction, and finally, a specific genotyping protocol is followed. Various researches are focused on overcoming these issues. Microcards off...
