Do, Tuyen T. H. Lai, Tran N. B. Stephenson, Steven L. Tran, Hanh T. M.
Published in
BMC Biotechnology
BackgroundMicrobial polysaccharides have been reported to possess remarkable bioactivities. Physarum polycephalum is a species of slime mold for which the microplasmodia are capable of rapid growth and can produce a significant amount of cell wall-less biomass. There has been a limited understanding of the polysaccharides produced by microplasmodia...
Cabre, L. Peyrard, S. Sirven, C. Gilles, L. Pelissier, B. Ducerf, S. Poussereau, N.
Published in
BMC Biotechnology
BackgroundPhakopsora pachyrhizi is a biotrophic fungal pathogen responsible for the Asian soybean rust disease causing important yield losses in tropical and subtropical soybean-producing countries. P. pachyrhizi triggers important transcriptional changes in soybean plants during infection, with several hundreds of genes being either up- or downreg...
Arevalos-Sánchez, Martha María Maynez-Perez, Adrián Omar Rodríguez-Almeida, Felipe A. Martínez-Quintana, José Alfredo Sanchez-Flores, Fidel Alejandro Felix-Portillo, Monserrath Chavéz-Martínez, América Olvera-García, Myrna Elena Ruiz-Barrera, Oscar Corral-Luna, Agustín
...
Published in
BMC Biotechnology
BackgroundThe production of agricultural wastes still growing as a consequence of the population growing. However, the majority of these residues are under-utilized due their chemical composition, which is mainly composed by cellulose. Actually, the search of cellulases with high efficiency to degrade this carbohydrate remains as the challenge. In ...
Xiong, Fei Cheng, Xiangyun Zhang, Chao Klar, Roland Manfred He, Tao
Published in
BMC Biotechnology
BackgroundReverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) remains one of the best-established techniques to assess gene expression patterns. However, appropriate reference gene(s) selection remains a critical and challenging subject in which inappropriate reference gene selction can distort results leading to false ...
Liebal, Ulf W Fabry, Brigida A Ravikrishnan, Aarthi Schedel, Constantin VL Schmitz, Simone Blank, Lars M Ebert, Birgitta E
Published in
BMC Biotechnology
BackgroundOgataea polymorpha is a thermotolerant, methylotrophic yeast with significant industrial applications. While previously mainly used for protein synthesis, it also holds promise for producing platform chemicals. O. polymorpha has the distinct advantage of using methanol as a substrate, which could be potentially derived from carbon capture...
Chen, Fan Li, Yi-ya Yu, Yan-li Dai, Jie Huang, Jin-ling Lin, Jie
Published in
BMC Biotechnology
BackgroundThe ability to clone DNA sequences quickly and precisely into plasmids is essential for molecular biology studies. The recent development of seamless cloning technologies has made significant improvements in plasmid construction, but simple and reliable tools are always desirable for time- and labor-saving purposes.ResultsWe developed and...
Adhami, Masoumeh Sadeghi, Balal Rezapour, Ali Haghdoost, Ali Akbar MotieGhader, Habib
Published in
BMC Biotechnology
BackgroundThe coronavirus disease-19 (COVID-19) emerged in Wuhan, China and rapidly spread worldwide. Researchers are trying to find a way to treat this disease as soon as possible. The present study aimed to identify the genes involved in COVID-19 and find a new drug target therapy. Currently, there are no effective drugs targeting SARS-CoV-2, and...
Chang, Chang Gong, Siyi Liu, Zhiping Yan, Qiaojuan Jiang, Zhengqiang
Published in
BMC Biotechnology
BackgroundProteases are important for hydrolysis of proteins to generate peptides with many bioactivities. Thus, the development of novel proteases with high activities is meaningful to discover bioactive peptides. Because natural isolation from animal, plant and microbial sources is impractical to produce large quantities of proteases, gene clonin...
Aslan, Cynthia Kiaie, Seyed Hossein Zolbanin, Naime Majidi Lotfinejad, Parisa Ramezani, Reihaneh Kashanchi, Fatah Jafari, Reza
Published in
BMC Biotechnology
Over the past decade, therapeutic messenger RNAs (mRNAs) have emerged as a highly promising new class of drugs for protein replacement therapies. Due to the recent developments, the incorporation of modified nucleotides in synthetic mRNAs can lead to maximizing protein expression and reducing adverse immunogenicity. Despite these stunning improveme...
Krahulec, Ján Šafránek, Martin
Published in
BMC Biotechnology
BackgroundThe aim of this study was to provide an information about the homogeneity on the level of enterokinase productivity in P. pastoris depending on different suppliers of the media components.ResultsIn previous studies, we performed the optimisation process for the production of enterokinase by improving the fermentation process. Enterokinase...
