Ma, Xiaodan Zhang, Liying Ren, Yijun Yun, Hui Cui, Hanlin Li, Qian Guo, Yuanqiang Gao, Shuhong Zhang, Fengliang Wang, Aijie
...
Published in
Applied and environmental microbiology
Antibiotic resistance mediated by bacterial enzyme inactivation plays a crucial role in the degradation of antibiotics in the environment. Chloramphenicol (CAP) resistance by enzymatic inactivation comprises nitro reduction, amide bond hydrolysis, and acetylation modification. However, the molecular mechanism of enzymatic oxidation of CAP remains u...
Dixon, Megan H. Cowles, Kimberly N. Zaacks, Sonia C. Marciniak, Isabel N. Barak, Jeri D.
Published in
Applied and Environmental Microbiology
The physiology of plant hosts can be dramatically altered by phytopathogens. Xanthomonas hortorum pv. gardneri is one such pathogen that creates an aqueous niche within the leaf apoplast by manipulating the plant via the transcription activator-like effector AvrHah1. Simultaneous immigration of X. hortorum pv. gardneri and Salmonella enterica to he...
Sullivan, Genevieve Orsi, Renato H. Estrada, Erika Strawn, Laura Wiedmann, Martin
Published in
Applied and Environmental Microbiology
The contamination of ready-to-eat produce with Listeria monocytogenes (LM) can often be traced back to environmental sources in processing facilities and packinghouses. To provide an improved understanding of Listeria sources and transmission in produce operations, we performed whole-genome sequencing (WGS) of LM (n = 169) and other Listeria spp. (...
Cha, Guihong Liu, Yugeng Yang, Qing Bai, Liping Cheng, Lei Fan, Wei
Published in
Applied and Environmental Microbiology
Diverse archaea, including many unknown species and phylogenetically deeply rooted taxa, survive in extreme environments. They play crucial roles in the global carbon cycle and element fluxes in many terrestrial, marine, saline, host-associated, hot-spring, and oilfield environments. There is little knowledge of the diversity of chemoreceptors that...
Yuan, Chenyang Song, Shikui He, Jianxin Zhang, Jingxian Liu, Xiangcen Pena, Eliana L. Sun, Junsong Shi, Jiping Su, Zhengding Zhang, Baoguo
...
Published in
Applied and Environmental Microbiology
Steroid drug precursors, including C19 and C22 steroids, are crucial to steroid drug synthesis and development. However, C22 steroids are less developed due to the intricacy of the steroid metabolic pathway. In this study, a C22 steroid drug precursor, 9-hydroxy-3-oxo-4,17-pregadiene-20-carboxylic acid methyl ester (9-OH-PDCE), was successfully obt...
Martinez, Julien Ross, Perran A. Gu, Xinyue Ant, Thomas H. Murdochy, Shivan M. Tong, Lily da Silva Filipe, Ana Hoffmann, Ary A. Sinkins, Steven P.
Published in
Applied and Environmental Microbiology
The intracellular bacterium Wolbachia inhibits virus replication and is being harnessed around the world to fight mosquito-borne diseases through releases of mosquitoes carrying the symbiont. Wolbachia strains vary in their ability to invade mosquito populations and suppress viruses in part due to differences in their density within the insect and ...
Akob, Denise M. Sutton, John M. Bushman, Timothy J. Baesman, Shaun M. Klein, Edina Shrestha, Yesha Andrews, Robert Fierst, Janna L. Kolton, Max Gushgari-Doyle, Sara
...
Published in
Applied and Environmental Microbiology
Acetylene (C2H2) is a molecule rarely found in nature, with very few known natural sources, but acetylenotrophic microorganisms can use acetylene as their primary carbon and energy source. As of 2018 there were 15 known strains of aerobic and anaerobic acetylenotrophs; however, we hypothesize there may yet be unrecognized diversity of acetylenotrop...
Hon, Shuen Jacobson, Tyler Stevenson, David M. Maloney, Marybeth I. Giannone, Richard J. Hettich, Robert L. Amador-Noguez, Daniel Olson, Daniel G. Lynd, Lee R.
Published in
Applied and Environmental Microbiology
Glycolysis is an ancient, widespread, and highly conserved metabolic pathway that converts glucose into pyruvate. In the canonical pathway, the phosphofructokinase (PFK) reaction plays an important role in controlling flux through the pathway. Clostridium thermocellum has an atypical glycolysis and uses pyrophosphate (PPi) instead of ATP as the pho...
Zhang, Ling Wu, Zhengyu Zhang, Rongjing Yuan, Junhua
Published in
Applied and Environmental Microbiology
FliL is present in nearly all flagellated bacterial species and is associated with the flagellar basal body. This protein was found to be important for the function of the flagellar motor, and its absence led to a variety of motility defects in several species. However, the specific function of FliL in Pseudomonas aeruginosa remains elusive. Here, ...
Jiang, Wankui Zhang, Mingliang Gao, Siyuan Zhu, Qian Qiu, Jiguo Yan, Xin Xin, Fengxue Jiang, Min Hong, Qing
Published in
Applied and Environmental Microbiology
The worldwide use of the carbamate insecticide carbofuran has caused considerable concern about its environmental fate. Degradation of carbofuran by Sphingobium sp. strain CFD-1 is initiated via the hydrolysis of its ester bond by carbamate hydrolase CehA to form carbofuran phenol. In this study, another carbofuran-degrading strain, Sphingobium sp....
