Golovliov, Igor Bäckman, Stina Granberg, Malin Salomonsson, Emelie Lundmark, Eva Näslund, Jonas Busch, Joseph D Birdsell, Dawn Sahl, Jason W Wagner, David M
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Published in
Applied and environmental microbiology
Francisella tularensis, causative agent of the zoonotic disease tularemia, can cause seasonal outbreaks of acute febrile illness in humans with disease peaks in late summer to autumn. Interestingly, its mechanisms for environmental persistence between outbreaks are poorly understood. One hypothesis is that F. tularensis forms biofilm in aquatic env...
Synefiaridou, Dimitra Veening, Jan-Willem
Published in
Applied and environmental microbiology
CRISPR-Cas systems provide bacteria and archaea with adaptive immunity against viruses and plasmids by detection and cleavage of invading foreign DNA. Modified versions of this system can be exploited as a biotechnological tool for precise genome editing at a targeted locus. Here, we developed a replicative plasmid that carries the CRISPR-Cas9 syst...
Tegegne, Henok Ayalew Koláčková, Ivana Florianová, Martina Wattiau, Pierre Gelbíčová, Tereza Boland, Cécile Madec, Jean-Yves Haenni, Marisa Karpíšková, Renáta
Published in
Applied and environmental microbiology
Methicillin-resistant Staphylococcus aureus (MRSA) presenting the spa type t899 is commonly associated with sequence type (ST) 9, but also increasingly linked to ST398. This study provides a genomic insight into the diversity of t899 isolates using cgMLST, SNP-based phylogeny and the description of selected antimicrobial resistance and virulence ma...
Schubert, Hannah Morley, Katy Puddy, Emma F Arbon, Robert Findlay, Jacqueline Mounsey, Oliver Gould, Virginia C Vass, Lucy Evans, Madeleine Rees, Gwen M
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Published in
Applied and environmental microbiology
Little is known about the drivers of critically important antibacterial resistance in species with zoonotic potential present on farms (e.g. CTX-M β-lactamase-positive Escherichia coli). We collected samples - monthly, between January 2017 and December 2018 - on 53 dairy farms in South West England along with data for 610 variables concerning antib...
Goh, Yong Jun Barrangou, Rodolphe
Published in
Applied and environmental microbiology
Diverse Lactobacillus strains are widely used as probiotic cultures in the dairy and dietary supplements industries, and specific strains such as Lactobacillus acidophilus NCFM have been engineered for the development of biotherapeutics. To expand the Lactobacillus manipulation toolbox with enhanced efficiency and ease, we present here a CRISPR-Spy...
Cohen, Joshua J Eichinger, Steven J Witte, Danae A Cook, Connor J Fidopiastis, Pat M Tepavčević, Jovanka Visick, Karen L
Published in
Applied and environmental microbiology
Vibrio species, including the squid symbiont Vibrio fischeri, become competent to take up DNA under specific conditions. For example, V. fischeri becomes competent when grown in the presence of chitin oligosaccharides or upon overproduction of the competence regulatory factor TfoX. While little is known about the regulatory pathway(s) that control ...
Lubbers, Ronnie J M Dilokpimol, Adiphol Visser, Jaap Hildén, Kristiina S Mäkelä, Miia R de Vries, Ronald P
Published in
Applied and environmental microbiology
Salicylic acid plays an important role in the plant immune response and its degradation is therefore important for plant pathogenic fungi. However, many non-pathogenic microorganisms can also degrade salicylic acid. In the filamentous fungi Aspergillus niger, two salicylic acid metabolic pathways have been suggested. The first pathway converts sali...
Harrand, Anna Sophia Guariglia-Oropeza, Veronica Skeens, Jordan Kent, David Wiedmann, Martin
Published in
Applied and environmental microbiology
Inoculation studies are important when assessing microbial survival and growth in food products. These studies typically involve the pre-growth of multiple strains of a target pathogen under a single condition; this emphasizes strain diversity. To gain a better understanding of the impacts of strain diversity ("Nature") and pre-growth conditions ("...
Palacios-Gorba, Carla Moura, Alexandra Leclercq, Alexandre Gómez-Martín, Ángel Gomis, Jesús Jiménez-Trigos, Estrella Mocé, María L Lecuit, Marc Quereda, Juan J
Published in
Applied and environmental microbiology
Listeria monocytogenes (Lm) is a major human and animal foodborne pathogen. However, data from environmental reservoirs remain scarce. Here, we used whole genome sequencing to characterize Listeria spp. isolates recovered over one year from wild animals in their natural habitats in Spain. Three different Listeria spp. [Lm (n=19), Listeria ivanovii ...
Deaven, Abigail M Ferreira, Christina M Reed, Elizabeth A Chen See, Jeremy R Lee, Nora A Almaraz, Eduardo Rios, Paula C Marogi, Jacob G Lamendella, Regina Zheng, Jie
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Published in
Applied and environmental microbiology
Freshwater can support the survival of the enteric pathogen Salmonella, though temporal Salmonella diversity in a large watershed has not been assessed. At 28 locations within the Susquehanna River basin, 10-liter samples were assessed in spring and summer over two years. Salmonella prevalence was 49%, and increased river discharge was the main dri...
