Ren, Yijun Zhang, Mingliang Gao, Siyuan Zhu, Qian Ke, Zhijian Jiang, Wankui Qiu, Jiguo Hong, Qing
Published in
Applied and Environmental Microbiology
Phenazines are an important class of secondary metabolites and are primarily named for their heterocyclic phenazine cores, including phenazine-1-carboxylic acid (PCA) and its derivatives, such as phenazine-1-carboxamide (PCN) and pyocyanin (PYO). Although several genes involved in the degradation of PCA and PYO have been reported so far, the geneti...
Chen, Yun Zhao, Mingming Lv, Mengli Lin, Min Wang, Jin Zuo, Kaijing
Published in
Applied and Environmental Microbiology
Reactive oxygen species (ROS) can cause destructive damage to biological macromolecules and protein dysfunction in bacteria. Methionine sulfoxide reductase (Msr) with redox-active Cys and/or seleno-cysteine (Sec) residues can restore physiological functions of the proteome, which is essential for oxidative stress tolerance of the extremophile Deino...
Chen, Zongchao Niu, Pengfei Ren, Xiaomei Han, Wenlong Shen, Ruyu Zhu, Min Yu, Yang Ding, Chan Yu, Shengqing
Published in
Applied and Environmental Microbiology
Riemerella anatipestifer is a major pathogenic agent of duck septicemic and exudative diseases. Recent studies have shown that the R. anatipestifer type IX secretion system (T9SS) is a crucial factor in bacterial virulence. The AS87_RS04190 protein was obviously missing from the secreted proteins of the T9SS mutant strain Yb2ΔgldM. A bioinformatic ...
Royer, Guilhem Dixit, Zoya Pédron, Jacques Pierrat, Gautier Demontant, Vanessa Berçot, Béatrice Rodriguez, Christophe Barny, Marie-Anne Jacquier, Hervé Woerther, Paul-Louis
...
Published in
Applied and Environmental Microbiology
Genus Pectobacterium bacteria include important agricultural pathogens. Pectobacterium versatile isolates contain a chromosome-borne beta-lactamase, PEC-1. This enzyme is the closest relative of TEM-1, a plasmid-borne beta-lactamase widespread in the Enterobacterales. We performed bioinformatics and phenotypic analyses to investigate the genetic an...
Schmidt, Matthias Pearson, Allison N Incha, Matthew R Thompson, Mitchell G Baidoo, Edward E K Kakumanu, Ramu Mukhopadhyay, Aindrila Shih, Patrick M Deutschbauer, Adam M Blank, Lars M
...
Published in
Applied and environmental microbiology
Pseudomonas putida KT2440 has long been studied for its diverse and robust metabolisms, yet many genes and proteins imparting these growth capacities remain uncharacterized. Using pooled mutant fitness assays, we identified genes and proteins involved in the assimilation of 52 different nitrogen containing compounds. To assay amino acid biosynthesi...
Wang, Yue Nan, Xuemei Zhao, Yiguang Jiang, Linshu Wang, Hui Zhang, Fan Hua, Dengke Liu, Jun Yao, Junhu Yang, Liang
...
Published in
Applied and environmental microbiology
The milk microbiota and mediated metabolites directly affect the health of the udder in dairy cows. Inulin, a dietary prebiotic, can modulate the profile of gastrointestinal microbiota. However, whether the inulin intake affects the milk microbial population and metabolites remains unknown. In this study, 40 subclinical mastitis (SCM) cows were ran...
Garcia, Carolina Y Seo, Hyesuk Sack, David A Zhang, Weiping
Published in
Applied and environmental microbiology
There are no vaccines licensed for enterotoxigenic Escherichia coli (ETEC), a leading bacterial cause of children's diarrhea and travelers' diarrhea. MecVax, a multivalent E. coli vaccine candidate composed of two epitope- and structure-based polyvalent proteins (toxoid fusion 3xSTaN12S-mnLTR192G/L211A and colonization factor antigen [CFA]/I/II/IV ...
Quintero-Blanco, Juan Jimenez, Juan Garzón, Andrés
Published in
Applied and environmental microbiology
Killer yeasts and their toxins have many potential applications in environmental, medical, and industrial biotechnology. The killer phenotype in Saccharomyces cerevisiae relies on the cytoplasmic persistence of two dsRNA viruses, L-A and M. M encodes the toxin, and L-A provides proteins for expression, replication, and capsids for both viruses. Yea...
Gaardløs, Margrethe Heggeset, Tonje Marita Bjerkan Tøndervik, Anne Tezé, David Svensson, Birte Ertesvåg, Helga Sletta, Håvard Aachmann, Finn Lillelund
Published in
Applied and environmental microbiology
The structure and functional properties of alginates are dictated by the monomer composition and molecular weight distribution. Mannuronan C-5-epimerases determine the monomer composition by catalyzing the epimerization of β-d-mannuronic acid (M) residues into α-l-guluronic acid (G) residues. The molecular weight is affected by alginate lyases, whi...
Huang, Ting Lu, Zhen-Ming Peng, Ming-Ye Chai, Li-Juan Zhang, Xiao-Juan Shi, Jin-Song Li, Qi Xu, Zheng-Hong
Published in
Applied and environmental microbiology
Mature vinegar culture has usually been used as a type of autochthonous starter to rapidly initiate the next batch of acetic acid fermentation (AAF) and maintain the batch-to-batch uniformity of AAF in the production of traditional cereal vinegar. However, the vitality and dominance of functional microbes in autochthonous starters remain unclear, w...
