Tan, Malcolm Turk Hsern Gong, Zhiyuan Li, Dan
Published in
Applied and Environmental Microbiology
This study reports an essential improvement of the method for replication of human norovirus (HNoV) with the use of zebrafish (Danio rerio) embryos. With three HNoV genotypes and P-types GII.2[P16], GII.4[P16], and GII.17[P31], we demonstrated that this tool had higher efficiency and robustness than the zebrafish larvae as reported previously. When...
Lin, Nuo Qiao Liang, Zhi Bin Wang, Hui Shan Wu, Xiao Yan Zhang, Lian Hui Deng, Yi Zhen
Published in
Applied and Environmental Microbiology
Sporisorium scitamineum and Ustilago maydis are two fungal pathogens causing severe sugarcane and maize diseases, respectively. Sexual mating of compatible sporidia is essential for these pathogens to form infections dikaryotic mycelia and cause smut diseases. We showed recently that in the presence of exogenous glucose, the Pseudomonas sp. strain ...
Gonzalez-Nayeck, Ana C. Grim, Sharon L. Waldbauer, Jacob Dick, Gregory J. Pearson, Ann
Published in
Applied and Environmental Microbiology
Modern microbial mats are potential analogues for Proterozoic ecosystems, yet only a few studies have characterized mats under low-oxygen conditions that are relevant to Proterozoic environments. Here, we use protein-stable isotope fingerprinting (P-SIF) to determine the protein carbon isotope (δ13C) values of autotrophic, heterotrophic, and mixotr...
Chang, Guanhong Luo, Zonghong Zhang, Yao Xu, Xu Zhou, Ting Wang, Xin
Published in
Applied and Environmental Microbiology
The purpose of this study was to evaluate the mechanism of sterilization of Staphylococcus aureus by electron beam irradiation (0.5-, 1-, 2-, 4-, and 6-kGy treatments) and whether it reduces the toxicity of its fermentation supernatant. In this study, we investigated the mechanism of sterilization of S. aureus by electron beam irradiation using col...
Nishiyama, Keita Yong, Cheng-Chung Moritoki, Nobuko Kitazawa, Haruki Odamaki, Toshitaka Xiao, Jin-Zhong Mukai, Takao
Published in
Applied and Environmental Microbiology
The human gastrointestinal tract is inhabited by trillions of symbiotic bacteria that form a complex ecological community and influence human physiology. Symbiotic nutrient sharing and nutrient competition are the most studied relationships in gut commensals, whereas the interactions underlying homeostasis and community maintenance are not fully un...
Sweet, Philip Blacutt, Jacob Gordon, Vernita Contreras, Lydia
Published in
Applied and Environmental Microbiology
Currently, there is a lack of bacterial biomarkers indicative of exposure to ionizing radiation (IR). IR biomarkers have applications for medical treatment planning, population exposure surveillance, and IR sensitivity studies. In this study, we compared the utility of signals originating from prophages and the SOS regulon as biomarkers of IR expos...
Li, Lili Zhang, Jie Zhou, Liyan Shi, Haijuan Mai, Hong Su, Junchang Ma, Xingwang Zhong, Jin
Published in
Applied and Environmental Microbiology
Vaginal infections continue to be a serious public health issue, and developing new approaches to address antibiotic-resistant pathogens is an urgent task. The dominant vaginal Lactobacillus species and their active metabolites (e.g., bacteriocins) have the potential to defeat pathogens and help individuals recover from disorders. Here, we describe...
Gao, Qilin Ma, Bingbing Wang, Qianwen Zhang, Hao Fushinobu, Shinya Yang, Jian Lin, Susu Sun, Keke Han, Bing-Nan Xu, Lian-Hua
...
Published in
Applied and Environmental Microbiology
Cytochrome P450 enzymes are promising biocatalysts for industrial use because they catalyze site-selective C–H oxidation and have diverse catalytic reactions and a broad substrate range. In this study, the 2α-hydroxylation activity of CYP154C2 from Streptomyces avermitilis MA-4680T toward androstenedione (ASD) was identified by an in vitro conversi...
González-Fernández, Adriana Symonds, Erin M. Gallard-Gongora, Javier F. Mull, Bonnie Lukasik, Jerzy O. Rivera Navarro, Pablo Badilla Aguilar, Andrei Peraud, Jayme Mora Alvarado, Darner Cantor, Allison
...
Published in
Applied and Environmental Microbiology
Population growth and changing climate are expected to increase human exposure to pathogens in tropical coastal waters. We examined microbiological water quality in three rivers within 2.3 km of each other that impact a Costa Rican beach and in the ocean outside their plumes during the rainy and dry seasons. We performed quantitative microbial risk...
Su, Lei Teske, Andreas P. MacGregor, Barbara J. McKay, Luke J. Mendlovitz, Howard Albert, Daniel Ma, Zhonglin Li, Jiangtao
Published in
Applied and Environmental Microbiology
The Guaymas Basin in the Gulf of California is characterized by active seafloor spreading, hydrothermal activity, and organic matter accumulation on the seafloor due to high sedimentation rates. In the hydrothermal sediments of Guaymas Basin, microbial community compositions and coexistence patterns change across steep gradients of temperature, pot...