Li, W. Tao, Y. Song, C. F. Feng, Y. D. Xie, J. Qian, Y. F.
Published in
Applied Biochemistry and Microbiology
AbstractThe codon-optimized zebrafish β-defensin 3 mature peptide gene mzfDB3 and channel catfish c-type lysozyme gene cflyC were used to design the fusion gene cflyC-mzfDB3. In the fusion protein cflyC-mzfDB3, 4×Gly flexible amino acid linker with an enterokinase cleavage site DDDDK was designed to link the mzfDB3 to the C-terminus of cflyC, which...
Khummanee, N. Rudeekulthamrong, P. Kaulpiboon, J.
Published in
Applied Biochemistry and Microbiology
AbstractXylose glucosides (XGs) have been effectively used in many industries because of their resistance to browning reactions and acids and prebiotic properties. In this study, XGs were synthesized through the transglycosylation reaction of recombinant amylomaltase. The optimal conditions for synthesis included the incubation in 1.5% (wt/vol) tap...
Thuoc, D. V. Anh, V. T. M.
Published in
Applied Biochemistry and Microbiology
AbstractThe biosynthesis of polyhydroxyalkanoates from crude fish oil by bacteria isolated from soil samples was investigated. More than 200 bacterial colonies were isolated, among them 15 isolates were selected and tested for their abilities to synthesize PHA from crude fish oil. Eight strains were found to produce the poly-3-hydroxybutyrate (PHB)...
Le, N. N. Phan, H. C. T. Dang, D. M. T. Dang, C. M.
Published in
Applied Biochemistry and Microbiology
AbstractMain application of microfluidic paper-based analytical devices (µPADs) is for low-cost and portable assays. In order to reduce the cost, miniaturization seems to be an effective way to save reagents, samples, materials, etc. Present study demonstrates that mini-µPADs based on nitrocellulose membrane with only 2 main channels can perform sa...
Gulevich, A. Yu. Skorokhodova, A. Yu. Debabov, V. G.
Published in
Applied Biochemistry and Microbiology
AbstractThe microaerobic synthesis of 3-hydroxybutyric acid by the Escherichia coli strain BOX3.1 ∆4 PL-atoB PL-tesB (MG1655 lacIQ, ∆ackA-pta, ∆poxB, ∆ldhA, ∆adhE, ∆fadE, PL-SDphi10-atoB, Ptrc-ideal-4-SDphi10-fadB, PL-SDphi10-tesB), which was previously directly engineered for the biosynthesis of the target compound from glucose through the reverse...
Shirokikh, I. G. Ogorodnikova, S. Yu. Nazarova, Ya. I. Shupletsova, O. N.
Published in
Applied Biochemistry and Microbiology
AbstractGenetic engineering of glycine betaine (GB) synthesis offers a new approach to improvements in the productivity and stress resistance of cultivated plants. The possible effect of the bacterial heterologous sequence on the reaction of plants under oxidative stress (OS) induced by 3 mM of hydrogen peroxide (H2O2) was determined. The independe...
Panferov, V. G. Safenkova, I. V. Zherdev, A. V. Dzantiev, B. B.
Published in
Applied Biochemistry and Microbiology
AbstractRecently, immunochromatographic assay (ICA) methods have found a wide range of uses in medical diagnostic, quality control, and consumer safety products because of their rapidity and methodological simplicity of testing; however, ICAs are usually less sensitive than other analytical methods. In this review, we survey modern methodological s...
Antonov, Yu. A. Zhuravleva, I. L.
Published in
Applied Biochemistry and Microbiology
AbstractThe interaction between lysozyme (Lys) and pectins with different net and local charges and with block (BD) and random (RD) distributions of methoxyl groups is studied via dynamic light scattering, turbidimetry, high-resolution optical microscopy, and electrophoretic mobility measurements. RD 16.2, RD 38.2, BD 16.9, and BD 33.1 form water-s...
Ning, Y. C. Yang, H. N. Li, N. Liu, Y. Wang, C. Y. Zhang, X. Liu, L. L. Weng, P. F. Wu, Z. F.
Published in
Applied Biochemistry and Microbiology
AbstractA novel fibrinolytic serine metalloproteinase gene (aprx-sw5) from Bacillus velezensis SW5 was heterologously expressed in Escherichia coli Trans(DE3). Sequence analysis indicated that the open reading frame of aprx-sw5 had 1329 bp, encoding a protein of 442 amino acid residues. In silico analysis described 3D structure of AprX-SW5 for the ...
Arlyapov, V. A. Yudina, N. Yu. Machulin, A. V. Alferov, V. A. Ponamoreva, O. N. Reshetilov, A. N.
Published in
Applied Biochemistry and Microbiology
A new approach has been developed for the formation of the biosensor recognition elements via the immobilization of microorganisms in layer-by-layer films in order to determine the biochemical oxygen demand (BOD). Bioreceptor elements of BOD-biosensors have been fabricated based on the bacterial microorganisms Paracoccus yeei,Pseudomonas veronii, a...
