Published in Applied Biochemistry and Microbiology
AbstractThis work describes a modification of the pRH2521 vector of the pRH2502/pRH2521 system for CRISPR-dCas9-mediated RNA interference. The modification enabled an increase in the cloning efficiency of guide RNA spacers. The ability of the modified pRH2502/pRH2521 system to suppress the transcription of certain genes was evaluated with the use o...
Published in Applied Biochemistry and Microbiology
AbstractThe halotolerant phosphate-solubilizing fungus Penicillium sp. NAUSF2 was isolated from rhizospheric soil in coastal regions of South Gujarat, India, and identified on the basis of morphological characteristics and internal transcribed spacer gene sequence (ITS1 and ITS4). The isolate solubilized rock phosphate up to 843 µM. There was drop ...
Published in Applied Biochemistry and Microbiology
AbstractThe synthesis of α-amylase AmyM3 from B. flexus 406 in the recombinant strain B. subtilis 168-28 was significantly repressed during submerged cultivation in nutrient medium supplemented with 1% glucose. Repression of α-amylase synthesis by glucose in B. subtilis cells is observed at the level of transcription and is mediated by the cataboli...
Published in Applied Biochemistry and Microbiology
AbstractUnder stressful conditions, including infection with phytopathogens, plants produce various compounds that are growth activators, e.g., hydroxycinnamic acids (HAs), including ferulic acid (FA). The role of HAs in the relationship of plants and endophytic bacteria has not been studied in detail. The effect of FA at a concentration of 1.0–100...
Published in Applied Biochemistry and Microbiology
AbstractThe best known polymers (alginate, carrageenan, and acrylamide) were examined for their potential use as carriers of mycelium cells of Aspergillus ochraceus VKM-F4104D, a producer of fibrinolytic protease activator of protein C in human blood plasma during its deep cultivation. The morpho-cytological features of the development of A. ochrac...
Published in Applied Biochemistry and Microbiology
AbstractAnaerobic production of pyruvic acid from glucose by recombinant Escherichia coli strains with impaired fermentation ability during respiration with nitrate as an external terminal electron acceptor was studied. During nitrate respiration in a minimal salt medium lacking ammonium ions, the core E. coli strain MG1655 ∆ackA-pta, ∆poxB, ∆ldhA,...
Published in Applied Biochemistry and Microbiology
AbstractThe use of specialized cultures of microorganisms and biological products based on them is the most acceptable way to solve such topical problems as an increase in plant yield and protection from disease, as well as the elimination of the consequences of pollution with oil and oil products. The use of microorganisms with a complex of useful...
Published in Applied Biochemistry and Microbiology
AbstractThe recombinant proteins E6-CBD and E7-CBD, which are antigens E6 and E7 of human papillomavirus type 16 (HPV16), were connected by a glycine-serine spacer with the cellulose-binding domain (CBD) of Anaerocellum thermophilum in preparations obtained via synthesis in a heterologous expression system in Escherichia coli. A simple, effective, ...
Published in Applied Biochemistry and Microbiology
AbstractNowadays, an alarming increase in antibiotics resistance have steered intensive research to explore new substitutes for ineffective antibiotics. This study aimed (1) to identify the purified antibacterial peptide isolated from amoebocyte lysate of the Asian horseshoe crab, Tachypleus gigas, (2) to compare potency of antibacterial activity o...
Published in Applied Biochemistry and Microbiology
AbstractMilbemycin, a group of 16-membered macrolide antibiotics produced by Streptomyces milbemycinicus, has been widely used as an insecticide and an anthelmintic. To enhance the production of milbemycin, ribosomal engineering with heterologous expression of a regulatory gene was used to screen for a high milbemycin-producing mutant. The mutant S...
