Published in Applied Biochemistry and Microbiology
AbstractPhytoalexin production has been extensively studied using multiple methods. However, this is the first report on the use of matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) to detect diterpenoid phytoalexins in fungal-infected Thai rice plants. In this study, we successfully detected diterpenoid phytoalexins...
Published in Applied Biochemistry and Microbiology
AbstractThe role of the outer membrane components of Yersinia pseudotuberculosis and Yersinia pestis in the adsorption of pseudotuberculous diagnostic bacteriophage (PDB) was assessed with two model bacteriophage-antigen systems, in which the target antigens were either in the solution or on the surface of polystyrene microspheres. It was establish...
Published in Applied Biochemistry and Microbiology
AbstractExtracellular protease SptA from the halophilic archaeon Natrinema sp. J7 was successfully expressed in Bacillus subtilis SCK6 using touchdown two-step PCR and plasmid multimer. Culture optimization was conducted to increase SptA protease activity 132-fold. It was shown that enzyme activity achieved the maximum activity at pH 8.0 and 55°C w...
Published in Applied Biochemistry and Microbiology
AbstractDinitrosyl iron complexes with glutathione ligands (GS-DNICs) are a physiological form of nitric oxide that is stored and transported in the organism. In addition, these complexes can act as antioxidants and antiradical agents. It was shown that GS-DNICs protect hemoglobin (Hb) from oxidative modification by peroxynitrite. They prevent the ...
Published in Applied Biochemistry and Microbiology
AbstractThe inheritance of allelic variants of aromatic alcohol dehydrogenase CADim (CAD intermediate) and their effect on the severity of plant traits in spring bread wheat (fluorescence of seedling slices, micromorphology, and chemical composition of tissues) were studied. The plants with contrast CADim genotypes (homozygotes +/+ and –/–) were us...
Published in Applied Biochemistry and Microbiology
AbstractSix of 11 strains of endophytic bacteria from pea (Pisum sativum L.) seeds were found in an aqueous medium of seedling-root growth under hydroculture conditions. It was shown that bacterial inoculates of Rhizobium leguminosarum bv. viceae, Pseudomonas syringae pv. pisi, Azotobacter chroococcum and Rhodococcus erythropolis had different effe...
Published in Applied Biochemistry and Microbiology
AbstractLactic acid has a privileged position in the family of carboxylic substances due to its several applications, including the production of biodegradable polymers such as polylactic acid in recent years. Thus, it has been considered one of the top 10 biorefinery chemicals by the United States Department of Energy. Lactic acid can be obtained ...
Published in Applied Biochemistry and Microbiology
AbstractNon-enzymatic glycation is a spontaneous and deleterious phenomenon that normally takes place inside human body and causes tissue damage. In the course of glycation reaction between glycating agents and biomolecules, several reactive perilous intermediates and finally heterogeneous group of compounds, advanced glycation end products (AGEs),...
Published in Applied Biochemistry and Microbiology
AbstractThe leaching of nonferrous metals from copper–zinc and copper–nickel sulfidic concentrates with a ferric sulfate solution obtained via microbial oxidation of ferrous sulfate was studied under different conditions (temperature, pulp density, and oxidant concentration). The highest concentration of nonferrous metals in the liquid phase was re...
Published in Applied Biochemistry and Microbiology
AbstractAs a natural, symbiotic consortium of bacteria and yeasts, kefir grains provide a unique source of new probiotic microorganisms. In this work, three new isolates of lactobacilli, Lactobacillus helveticus and two strains of Lacticaseibacillus paracasei, were isolated from kefir grains originating from the North Caucasus region of Russia, and...
