Popova, E. A. Kreyer, V. G. Komarevtsev, S. K. Shabunin, S. V. Osmolovskiy, A. A.
Published in
Applied Biochemistry and Microbiology
AbstractA new, nonglycosylated, serine proteinase capable of hydrolyzing collagen, elastin, and fibrin was isolated from a complex preparation of proteins formed by the micromycete A. ustus 1 under solid-state fermentation. The enzyme had a molecular weight of 33 kDa and a pI of 4.6. The pH-optimum (6.0) and temperature optimum (41°C) of the work o...
Eprintsev, A. T. Anokhina, G. B. Oya, P. S. Dedov, Ya. I.
Published in
Applied Biochemistry and Microbiology
AbstractThe dynamics of the activity of glutamate dehydrogenase (GDH, EC 1.4.1.2) during germination in corn leaves and scutes has been studied. It was found that changes in its activity are due to biochemical and molecular aspects of enzyme functioning. This was confirmed in a study of the relative level of transcripts of the gdh-1 and gdh-2 genes...
Tarasov, S. E. Plekhanova, Yu. V. Bykov, A. G. Kazakov, A. S. Vishnevskaya, M. V. Parunova, Yu. M. Gotovtsev, P. M. Reshetilov, A. N.
Published in
Applied Biochemistry and Microbiology
AbstractThe bioelectrochemical and spectral properties of immobilized Gluconacetobacter sucrofermentas VKPM B-11267 bacteria were studied in the presence and absence of multiwalled carbon nanotubes (MWCNTs). The obtained characteristics were compared with the characteristics of Gluconobacter oxydans, which are phylogenetically close to them and are...
Redondo, A. Wood, D. Amaral, S. Ferré, J. Goti, D. Bertran, J.
Published in
Applied Biochemistry and Microbiology
AbstractToxoplasmosis is a widespread zoonosis with an impact on immunocompromised people and critical in pregnant women because of its transmission to the fetus. Recombinant Toxoplasma gondii antigens produced in Escherichia coli are useful for antibody detection in patient’s blood. The aim of the study was to evaluate the feasibility of deriving ...
Mukhammadiev, R. S. Mukhammadiev, R. S. Skvortsov, E. V. Valiullin, L. R. Glinushkin, A. P. Bagaeva, T. V.
Published in
Applied Biochemistry and Microbiology
AbstractElectrophoretically homogeneous lectin with a specific activity of 100.0 U/mg protein was isolated and purified from the fungal mycelium of Fusarium solani 4. The molecular weight of the native lectin molecule (38 kDa) was determined. It was established that it consists of two identical subunits, with the molecular mass determined via PAGE-...
Wawro, A.
Published in
Applied Biochemistry and Microbiology
AbstractThe aim of this study was to obtain new distilling strains of Saccharomyces cerevisiae with increased resistance to acetic acid, and, at the same time, characterized by high ethanol production. The first stage of the research focused on the construction of a parental strains library (4 strains) by carrying out a chemical mutagenesis using e...
Palmina, N. P. Misharina, T. A. Krikunova, N. I. Antipova, A. S. Martirosova, E. I. Semenova, M. G.
Published in
Applied Biochemistry and Microbiology
AbstractGas chromatography was used to study changes in the fatty-acid (FA) profile of the liver and brain lipids in six groups of mice that received nanoliposomal complexes of different compositions added to drinks that replaced water in a long-term diet (3 months). The components of the six nanoliposomal types included clove essential oil (CEO), ...
Trutneva, K. A. Avdienko, V. G. Demina, G. R. Shleeva, M. O. Shumkov, M. S. Salina, E. G. Kaprelyants, A. S.
Published in
Applied Biochemistry and Microbiology
AbstractThe protein profile of dormant Mtb obtained after the gradual acidification of Mtb culture was studied to find antigenic proteins for humans that are expressed by M. tuberculosis (Mtb) cells in vitro under conditions close to the situation of persistence in vivo. According to 2D electrophoresis, a significant diversity of proteins in dorman...
Frolova, G. M. Kotlova, E. R. Sokornova, S. V. Senik, S. V. Shavarda, A. L. Misharev, A. D. Berestetskiy, A. O.
Published in
Applied Biochemistry and Microbiology
AbstractIn order to identify qualitative markers of a potential mycoherbicide against Canada thistle based on the mycelium of the fungus Stagonospora cirsii VIZR 1.41, changes in its pathogenicity and lipid profiles during submerged cultivation on liquid sucrose-soybean medium were analyzed. The fatty-acid composition of major lipids and the identi...
Korotkov, E. V. Yakovleva, I. V. Kamionskaya, A. M.
Published in
Applied Biochemistry and Microbiology
AbstractIn Russia and around the world, there are important questions regarding the potential threats to national and biological safety created by genetic technologies and the need to improve or introduce new, justified, and adequate measures for their control, regulation, and prevention. The article shows that a significant volume of the global ma...
