Wei, Ji-Ping Wang, Heng Luo, Tao Zhou, Zhi-Jiang Huang, Yan-Feng Qiao, Bin
Published in
Analytical and Bioanalytical Chemistry
Highly efficient extraction of peptides from serum is critical for finding serum biomarkers using mass spectrometry, which still remains a great challenge. Currently, a bottom-up proteomics approach has been applied to discover serum biomarkers. However, the approach was labor intensive, time and cost consuming, and cannot meet the requirements for...
Badran, Ahmed Ali Morais, Sergi Maquieira, Ángel
Published in
Analytical and Bioanalytical Chemistry
A multiplex competitive microimmunoassay for the simultaneous determination of gliadin, casein, β-lactoglobulin, and ovalbumin is presented. The assay in microarray format is performed on a DVD where the allergens are physisorbed on the polycarbonate surface of the disc. The immunointeraction is detected using a mixture of specific gold-labeled ant...
Cotte, Jean-François Bouadam, Afifa Sordoillet, Agathe Jaudinaud, Isabelle Chambon, Véronique Talaga, Philippe
Published in
Analytical and Bioanalytical Chemistry
Synthetic polyelectrolytes are a broad class of vaccine adjuvants. Among them, polyacrylic acid (PAA), a polyanionic polymer, is currently evaluated by Sanofi Pasteur. As chain length is considered to be a critical quality attribute for adjuvant properties of PAA, measurement of precise and accurate molecular size parameters is important for these ...
Li, Yaping Xu, Tao Chen, Xiaomei Lin, Shin Cho, Michael Sun, Dong Yang, Mengsu
Published in
Analytical and Bioanalytical Chemistry
Tumor metastasis is the primary cause of cancer death. Numerous studies have demonstrated the electrotactic responses of various cancer cell types, and suggested its potential implications in metastasis. In this study, we used a microfluidic device to emulate endogenous direct current electric field (dcEF) environment, and studied the electrotactic...
Raymond, Carolyn A. Davies, Noel W. Larkman, Tony
Published in
Analytical and Bioanalytical Chemistry
Tea tree oil distilled from Melaleuca alternifolia has widespread use in the cosmetic industry as an antimicrobial as well as for other functions in topical products. Concerns were first raised by the European Commission’s Scientific Committee on Consumer Products in 2004 about the level of the potentially carcinogenic phenylpropanoid compound meth...
Ringling, Christiane Rychlik, Michael
Published in
Analytical and Bioanalytical Chemistry
To investigate the often reported disagreement in food folate quantitation between the microbiological assay and high-performance liquid chromatography methods, different foods were analyzed both by a microbiological assay and by a liquid chromatography–tandem mass spectrometry (LC–MS/MS) method. For the LC–MS/MS analysis we emphasize the need for ...
de la Mata, A. Paulina McQueen, Rachel H. Nam, Seo Lin Harynuk, James J.
Published in
Analytical and Bioanalytical Chemistry
Human axillary sweat is a poorly explored biofluid within the context of metabolomics when compared to other fluids such as blood and urine. In this paper, we explore the volatile organic compounds emitted from two different types of fabric samples (cotton and polyester) which had been worn repeatedly during exercise by participants. Headspace soli...
Rebois, Rolando Onidas, Delphine Marcott, Curtis Noda, Isao Dazzi, Alexandre
Published in
Analytical and Bioanalytical Chemistry
Poly[(R)-3-hydroxyalkanoate]s or PHAs are aliphatic polyesters produced by numerous microorganisms. They are accumulated as energy and carbon reserve in the form of small intracellular vesicles. Poly[(R)-3-hydroxybutyrate] (PHB) is the most ubiquitous and simplest PHA. An atomic force microscope coupled with a tunable infrared laser (AFM-IR) was us...
Chen, Kuan-Ling Jiang, Shiuh-Jen Chen, Yen-Ling
Published in
Analytical and Bioanalytical Chemistry
International limits have been established for metal impurities in cosmetics to prevent overexposure to heavy metal ions. Sweeping via dynamic chelation was developed using capillary electrophoresis to analyze lead (Pb), cadmium (Cd) and mercury (Hg) impurities in cosmetics. The sweeping via dynamic chelation mechanism involves a large volume of me...
Pitarch-Andrés, Susana Roca-Genovés, Pasqual Peris-Vicente, Juan Esteve-Romero, Josep
Published in
Analytical and Bioanalytical Chemistry
An analytical method for the quantification of the herbicides and algaecides diuron, terbuthylazine, and terbutryn in wastewater and soil by micellar liquid chromatography was developed. The sample preparation was expedited to reduce the number of intermediate steps and the use of chemicals. The analytes in soils were recovered by ultrasonication i...