Kozak, Sandra Bloch, Yehudi De Munck, Steven Mikula, Aleksandra Bento, Isabel Savvides, Savvas N Meijers, Rob
Published in
Acta crystallographica. Section D, Structural biology
Structural studies of glycoproteins and their complexes provide critical insights into their roles in normal physiology and disease. Most glycoproteins contain N-linked glycosylation, a key post-translation modification that critically affects protein folding and stability and the binding kinetics underlying protein interactions. However, N-linked ...
Mirgaux, Manon Leherte, Laurence Wouters, Johan
Published in
Acta crystallographica. Section D, Structural biology
Indoleamine 2,3-dioxygenase 1 has sparked interest as an immunotherapeutic target in cancer research. Its structure includes a loop, named the JK-loop, that controls the orientation of the substrate or inhibitor within the active site. However, little has been reported about the crystal structure of this loop. In the present work, the conformation ...
Cowtan, K Metcalfe, S Bond, P
Published in
Acta crystallographica. Section D, Structural biology
The aim of crystallographic structure solution is typically to determine an atomic model which accurately accounts for an observed diffraction pattern. A key step in this process is the refinement of the parameters of an initial model, which is most often determined by molecular replacement using another structure which is broadly similar to the st...
Mehr, Alexander Henneberg, Fabian Chari, Ashwin Görlich, Dirk Huyton, Trevor
Published in
Acta crystallographica. Section D, Structural biology
The growth of diffraction-quality crystals and experimental phasing remain two of the main bottlenecks in protein crystallography. Here, the high-affinity copper(II)-binding tripeptide GHK was fused to the N-terminus of a GFP variant and an MBP-FG peptide fusion. The GHK tag promoted crystallization, with various residues (His, Asp, His/Pro) from s...
Hiromoto, Takeshi Nishikawa, Koji Inoue, Seiya Matsuura, Hiroaki Hirano, Yu Kurihara, Kazuo Kusaka, Katsuhiro Cuneo, Matthew Coates, Leighton Tamada, Taro
...
Published in
Acta crystallographica. Section D, Structural biology
A membrane-bound hydrogenase from Desulfovibrio vulgaris Miyazaki F is a metalloenzyme that contains a binuclear Ni-Fe complex in its active site and mainly catalyzes the oxidation of molecular hydrogen to generate a proton gradient in the bacterium. The active-site Ni-Fe complex of the aerobically purified enzyme shows its inactive oxidized form, ...
Masmaliyeva, Rafiga C Babai, Kave H Murshudov, Garib N
Published in
Acta crystallographica. Section D, Structural biology
This paper describes the global and local analysis of atomic displacement parameters (ADPs) of macromolecules in X-ray crystallography. The distribution of ADPs is shown to follow the shifted inverse-gamma distribution or a mixture of these distributions. The mixture parameters are estimated using the expectation-maximization algorithm. In addition...
Keown, Jeremy R Yang, Joy Black, Moyra M Goldstone, David C
Published in
Acta crystallographica. Section D, Structural biology
Members of the TRIM protein family have been shown to inhibit a range of viral infections. Recently, TRIM69 was identified as a potent inhibitor of Vesicular stomatitis Indiana virus infection, with its inhibition being dependent upon multimerization. Using SEC-MALLS analysis, it is demonstrated that the assembly of TRIM69 is mediated through the R...
Raj, Prateek Karthik, S Arif, S M Varshney, U Vijayan, M
Published in
Acta crystallographica. Section D, Structural biology
Mycobacterium smegmatis MutT1 (MsMutT1) is a sanitation enzyme made up of an N-terminal Nudix hydrolase domain and a C-terminal domain resembling a histidine phosphatase. It has been established that the action of MutT1 on 8-oxo-dGTP, 8-oxo-GTP and diadenosine polyphosphates is modulated by intermolecular interactions. In order to further explore t...
Ikeda, Akihito Urata, Seia Ando, Tadashi Suzuki, Yasuhiro Sato, Yasufumi Nishino, Tatsuya
Published in
Acta crystallographica. Section D, Structural biology
Vasohibins regulate angiogenesis, tumor growth, metastasis and neuronal differentiation. They form a complex with small vasohibin-binding protein (SVBP) and show tubulin tyrosine carboxypeptidase activity. Recent crystal structure determinations of vasohibin-SVBP complexes have provided a molecular basis for complex formation, substrate binding and...
Eriksson, Axl Caldararu, Octav Ryde, Ulf Oksanen, Esko
Published in
Acta crystallographica. Section D, Structural biology
The structure and function of proteins are strongly affected by the surrounding solvent water, for example through hydrogen bonds and the hydrophobic effect. These interactions depend not only on the position, but also on the orientation, of the water molecules around the protein. Therefore, it is often vital to know the detailed orientations of th...
