Hilario, Eduardo De Keyser, Sawyer Fan, Li
Published in
Acta crystallographica. Section D, Structural biology
The genus Xanthomonas comprises several cosmopolitan plant-pathogenic bacteria that affect more than 400 plant species, most of which are of economic interest. Citrus canker is a bacterial disease that affects citrus species, reducing fruit yield and quality, and is caused by the bacterium Xanthomonas citri subsp. citri (Xac). The Xac3819 gene, whi...
Richards, Logan S Millán, Claudia Miao, Jennifer Martynowycz, Michael W Sawaya, Michael R Gonen, Tamir Borges, Rafael J Usón, Isabel Rodriguez, Jose A
Published in
Acta crystallographica. Section D, Structural biology
Structure determination of novel biological macromolecules by X-ray crystallography can be facilitated by the use of small structural fragments, some of only a few residues in length, as effective search models for molecular replacement to overcome the phase problem. Independence from the need for a complete pre-existing model with sequence similar...
Weis, Felix Hagen, Wim J H
Published in
Acta crystallographica. Section D, Structural biology
Cryo-electron microscopy (cryo-EM) can be used to elucidate the 3D structure of macromolecular complexes. Driven by technological breakthroughs in electron-microscope and electron-detector development, coupled with improved image-processing procedures, it is now possible to reach high resolution both in single-particle analysis and in cryo-electron...
Gavira, Jose A Rodriguez-Ruiz, Isaac Martinez-Rodriguez, Sergio Basu, Shibom Teychené, Sébastien McCarthy, Andrew A Mueller-Dieckman, Christoph
Published in
Acta crystallographica. Section D, Structural biology
Sample handling and manipulation for cryoprotection currently remain critical factors in X-ray structural determination. While several microchips for macromolecular crystallization have been proposed during the last two decades to partially overcome crystal-manipulation issues, increased background noise originating from the scattering of chip-fabr...
Hishiki, Asami Sato, Mamoru Hashimoto, Hiroshi
Published in
Acta crystallographica. Section D, Structural biology
HLTF (helicase-like transcription factor) is a yeast RAD5 homolog that is found in mammals. HLTF has E3 ubiquitin ligase and DNA helicase activities, and is a pivotal protein in template-switched DNA synthesis that allows DNA replication to continue even in the presence of DNA damage by utilizing a newly synthesized undamaged strand as a template. ...
Bond, Paul S Wilson, Keith S Cowtan, Kevin D
Published in
Acta crystallographica. Section D, Structural biology
Manually identifying and correcting errors in protein models can be a slow process, but improvements in validation tools and automated model-building software can contribute to reducing this burden. This article presents a new correctness score that is produced by combining multiple sources of information using a neural network. The residues in 639...
Blank, P N Barnett, A A Ronnebaum, T A Alderfer, K E Gillott, B N Christianson, D W Himmelberger, J A
Published in
Acta crystallographica. Section D, Structural biology
Archaea are uniquely adapted to thrive in harsh environments, and one of these adaptations involves the archaeal membrane lipids, which are characterized by their isoprenoid alkyl chains connected via ether linkages to glycerol 1-phosphate. The membrane lipids of the thermophilic and acidophilic euryarchaeota Thermoplasma volcanium are exclusively ...
Wolf, Nina M Lee, Hyun Zagal, Daniel Nam, Joo Won Oh, Dong Chan Lee, Hanki Suh, Joo Won Pauli, Guido F Cho, Sanghyun Abad-Zapatero, Celerino
...
Published in
Acta crystallographica. Section D, Structural biology
The biological processes related to protein homeostasis in Mycobacterium tuberculosis, the etiologic agent of tuberculosis, have recently been established as critical pathways for therapeutic intervention. Proteins of particular interest are ClpC1 and the ClpC1-ClpP1-ClpP2 proteasome complex. The structure of the potent antituberculosis macrocyclic...
Baker, Edward N
Published in
Acta crystallographica. Section D, Structural biology
Sader, Kasim Matadeen, Rishi Castro Hartmann, Pablo Halsan, Tor Schlichten, Chris
Published in
Acta crystallographica. Section D, Structural biology
Cryo-electron microscopy (cryo-EM) has rapidly expanded with the introduction of direct electron detectors, improved image-processing software and automated image acquisition. Its recent adoption by industry, particularly in structure-based drug design, creates new requirements in terms of reliability, reproducibility and throughput. In 2016, Therm...
