Oliveira, Gabriel S de Dos S Bury, Priscila Huang, Fanglu Li, Yuan Araújo, Natália C de Zhou, Jiahai Sun, Yuhui Leeper, Finian J Leadlay, Peter F Dias, Marcio V B
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Published in
ACS chemical biology
Aminoglycosides are essential antibiotics used to treat severe infections caused mainly by Gram-negative bacteria. Gentamicin is an aminoglycoside and, despite its toxicity, is clinically used to treat several pulmonary and urinary infections. The commercial form of gentamicin is a mixture of five compounds with minor differences in the methylation...
Wang, Kwo-Kwang Abraham Singh, Jupneet Albin, John S Pentelute, Bradley L Nolan, Elizabeth M
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ACS chemical biology
Dysbiosis of the human gut microbiota is linked to numerous diseases. Understanding the molecular mechanisms by which microbes interact and compete with one another is required for developing successful strategies to modulate the microbiome. The natural product Microcin M (MccM) consists of a 77-residue bioactive peptide conjugated to a siderophore...
Kobayashi, Ryo Saito, Keita Kodani, Shinya
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ACS chemical biology
The class III lanthipeptide synthetase (LanKC) installs unusual amino acids, such as lanthionine and labionin, in lanthipeptides. Through genome mining, we discovered a new class III lanthipeptide synthetase coding gene (nptKC) and precursor peptide coding genes (nptA1, nptA2, and nptA3) in the genome of the actinobacterium Nocardiopsis alba. Coexp...
Tenney, Logan Pham, Vanha N Chang, Christopher J
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ACS chemical biology
Formaldehyde is commonly thought of as an environmental toxin or laboratory fixation reagent, but there is a growing appreciation for its broader physiological contributions as a naturally generated one-carbon metabolite across all kingdoms of life. In this In Focus article, we summarize emerging advances in the field that show how formaldehyde pla...
Chen, Yuqi Simeone, Angela Melidis, Larry Cuesta, Sergio Martinez Tannahill, David Balasubramanian, Shankar
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ACS chemical biology
Four-stranded G-quadruplexes (G4s) are DNA secondary structures that can form in the human genome. G4 structures have been detected in gene promoters and are associated with transcriptionally active chromatin and the recruitment of transcription factors and chromatin remodelers. We adopted a controlled, synthetic biology approach to understand how ...
Yu, Miao Tang, T M Simon Ghamsari, Lila Yuen, Graham Scuoppo, Claudio Rotolo, Jim A Kappel, Barry J Mason, Jody M
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ACS chemical biology
Activating transcription factor 3 (ATF3) is an activation transcription factor/cyclic adenosine monophosphate (cAMP) responsive element-binding (CREB) protein family member. It is recognized as an important regulator of cancer progression by repressing expression of key inflammatory factors such as interferon-γ and chemokine (C-C motif) ligand 4 (C...
Peng, Huiyun Schmiederer, Julian Chen, Xiuqiang Panagiotou, Gianni Kries, Hajo
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ACS chemical biology
Nonribosomal peptide synthetases (NRPSs) are sophisticated molecular machines that biosynthesize peptide drugs. In attempts to generate new bioactive compounds, some parts of NRPSs have been successfully manipulated, but especially the influence of condensation (C-)domains on substrate specificity remains enigmatic and poorly controlled. To underst...
Guzmán, Lindsay E Cambier, C J Cheng, Tan-Yun Naqvi, Kubra F Shiloh, Michael U Moody, D Branch Bertozzi, Carolyn R
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ACS chemical biology
Surface lipids on pathogenic mycobacteria modulate infection outcomes by regulating host immune responses. Phenolic glycolipid (PGL) is a host-modulating surface lipid that varies among clinical Mycobacterium tuberculosis strains. PGL is also found in Mycobacterium marinum, where it promotes infection of zebrafish through effects on the innate immu...
Castro-Falcón, Gabriel Straetener, Jan Bornikoel, Jan Reimer, Daniela Purdy, Trevor N Berscheid, Anne Schempp, Florence M Liu, Dennis Y Linington, Roger G Brötz-Oesterhelt, Heike
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Published in
ACS chemical biology
Elucidating the mechanism of action (MoA) of antibacterial natural products is crucial to evaluating their potential as novel antibiotics. Marinopyrroles, pentachloropseudilin, and pentabromopseudilin are densely halogenated, hybrid pyrrole-phenol natural products with potent activity against Gram-positive bacterial pathogens like Staphylococcus au...
Gu, Yushu Liu, Miaomiao Ma, Linlin Quinn, Ronald J
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ACS chemical biology
The inwardly rectifying potassium Kir4.2 channel plays a crucial role in regulating membrane potentials and maintaining potassium homeostasis. Kir4.2 has been implicated in various physiological processes, including insulin secretion, gastric acid regulation, and the pathogenesis of central nervous system diseases. Despite its significance, the num...