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X-ray crystal structures of 70S ribosome functional complexes.

Authors
  • Jh, Cate
  • Mm, Yusupov
  • Gz, Yusupova
  • Tn, Earnest
  • Harry Noller
Type
Published Article
Journal
Science
Publisher
American Association for the Advancement of Science (AAAS)
Volume
285
Issue
5436
Pages
2095–2104
Source
UCSC Bioinformatics biomedical-ucsc
License
Unknown

Abstract

Structures of 70S ribosome complexes containing messenger RNA and transfer RNA (tRNA), or tRNA analogs, have been solved by x-ray crystallography at up to 7.8 angstrom resolution. Many details of the interactions between tRNA and the ribosome, and of the packing arrangements of ribosomal RNA (rRNA) helices in and between the ribosomal subunits, can be seen. Numerous contacts are made between the 30S subunit and the P-tRNA anticodon stem-loop; in contrast, the anticodon region of A-tRNA is much more exposed. A complex network of molecular interactions suggestive of a functional relay is centered around the long penultimate stem of 16S rRNA at the subunit interface, including interactions involving the "switch" helix and decoding site of 16S rRNA, and RNA bridges from the 50S subunit.

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