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Wounding activates a 47 kDa MAP kinase in Catharanthus roseus (L.) G. Don.

Authors
  • Raina, Susheel Kumar
Type
Published Article
Journal
Indian journal of experimental biology
Publication Date
Feb 01, 2017
Volume
55
Issue
2
Pages
107–112
Identifiers
PMID: 30184411
Source
Medline
Language
English
License
Unknown

Abstract

Catharanthus roseus (L.) G. Don. is an important medicinal plant (Fam. Apocynaceae) known for its alkaloids that are accumulated in response to various biotic and abiotic stresses including wounding. Mitogen activated protein (MAP) kinases are important components of cellular signaling system transducing these stress stimuli into intracellular responses by phosphorylation of downstream transcription factors, regulators or inhibitors leading to expression of stress responsive genes. Here, we report the activation of a 47 kDa MAP Kinase in C. roseus in response to wounding. The immunoprecipitation coupled with in-gel kinase assay revealed tyrosine phosphorylation of the protein. Partial purification of the MAP kinase was also attempted. We observed a size variation for the kinase when purified using different schemes.

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