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WNT5A supports viability of senescent human dental follicle cells

Authors
  • Morsczeck, Christian1
  • Reck, Anja1
  • Reichert, Torsten E.1
  • 1 University Hospital Regensburg, Department of Oral and Maxillofacial Surgery, Franz-Josef-Strauss-Allee 11, Regensburg, 93053, Germany , Regensburg (Germany)
Type
Published Article
Journal
Molecular and Cellular Biochemistry
Publisher
Springer-Verlag
Publication Date
Nov 07, 2018
Volume
455
Issue
1-2
Pages
21–28
Identifiers
DOI: 10.1007/s11010-018-3467-9
Source
Springer Nature
Keywords
License
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Abstract

The osteogenic differentiation of dental follicle cells (DFCs) is inhibited by the onset of cellular senescence, but the cause for this is largely unknown. Recently it was shown that WNT5a, which is an inductor of the non-canonical WNT pathway, stimulates both cellular senescence and osteogenic differentiation of different cell types. In this study, we investigated the role of WNT5a for viability and osteogenic differentiation in human DFCs after the induction of cellular senescence. DFCs were cultivated until the induction of cellular senescence. The induction of cellular senescence was confirmed by β-galactosidase staining, estimation of population doubling time, and slightly telomere length shortening. After induction of cellular senescence, the expression of WNT5A and the potential to induce the osteogenic differentiation decreased. Inhibition of WNT5A by specific siRNAs had significant effect on the viability of DFCs. Cell proliferation was reduced, whereas both cellular senescence and cell death were increased in DFCs. However, an inhibition of WNT5A did only slightly effect the osteogenic differentiation of DFCs. Our results suggest that WNT5A supports viability during both cell proliferation and osteogenic differentiation of DFCs.

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