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Wnt produced by stretched roof-plate cells is required for the promotion of cell proliferation around the central canal of the spinal cord.

Authors
  • Shinozuka, Takuma1, 2, 3
  • Takada, Ritsuko1, 2
  • Yoshida, Shosei2, 3
  • Yonemura, Shigenobu4, 5
  • Takada, Shinji6, 2, 3
  • 1 Exploratory Research Center on Life and Living Systems, National Institutes of Natural Sciences, 5-1 Higashiyama, Myodaiji, Okazaki, Aichi 444-8787, Japan. , (Japan)
  • 2 National Institute for Basic Biology, National Institutes of Natural Sciences, 5-1 Higashiyama, Myodaiji, Okazaki, Aichi 444-8787, Japan. , (Japan)
  • 3 Department of Basic Biology in the School of Life Science, The Graduate University for Advanced Studies (SOKENDAI), 5-1 Higashiyama, Myodaiji, Okazaki, Aichi 444-8787, Japan. , (Japan)
  • 4 RIKEN Center for Life Science Technologies, 2-2-3 Minatojima-minamimachi, Chuo-ku, Kobe, Hyogo 650-0047, Japan. , (Japan)
  • 5 Department of Cell Biology, Tokushima University Graduate School of Medical Science, 3-18-15, Kuramoto-cho, Tokushima 770-8503, Japan. , (Japan)
  • 6 Exploratory Research Center on Life and Living Systems, National Institutes of Natural Sciences, 5-1 Higashiyama, Myodaiji, Okazaki, Aichi 444-8787, Japan [email protected] , (Japan)
Type
Published Article
Journal
Development
Publisher
The Company of Biologists
Publication Date
Jan 16, 2019
Volume
146
Issue
2
Identifiers
DOI: 10.1242/dev.159343
PMID: 30651295
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Cell morphology changes dynamically during embryogenesis, and these changes create new interactions with surrounding cells, some of which are presumably mediated by intercellular signaling. However, the effects of morphological changes on intercellular signaling remain to be fully elucidated. In this study, we examined the effect of morphological changes in Wnt-producing cells on intercellular signaling in the spinal cord. After mid-gestation, roof-plate cells stretched along the dorsoventral axis in the mouse spinal cord, resulting in new contact at their tips with the ependymal cells that surround the central canal. Wnt1 and Wnt3a were produced by the stretched roof-plate cells and delivered to the cell process tip. Whereas Wnt signaling was activated in developing ependymal cells, Wnt activation in dorsal ependymal cells, which were close to the stretched roof plate, was significantly suppressed in embryos with roof plate-specific conditional knockout of Wls, which encodes a factor that is essential for Wnt secretion. Furthermore, proliferation of these cells was impaired in Wls conditional knockout mice during development and after induced spinal cord injury in adults. Therefore, morphological changes in Wnt-producing cells appear to generate new Wnt signal targets. © 2019. Published by The Company of Biologists Ltd.

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