Affordable Access

Publisher Website

Differentiation of Hedyotis diffusa and Common Adulterants Based on Chloroplast Genome Sequencing and DNA Barcoding Markers.

Authors
  • Yik, Mavis Hong-Yu1
  • Kong, Bobby Lim-Ho1, 2
  • Siu, Tin-Yan2
  • Lau, David Tai-Wai1, 2
  • Cao, Hui3
  • Shaw, Pang-Chui1, 2, 4
  • 1 Li Dak Sum Yip Yio Chin R & D Center for Chinese Medicine, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong, China. , (China)
  • 2 Shiu-Ying Hu Herbarium, School of Life Sciences, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong, China. , (China)
  • 3 Research Center for Traditional Chinese Medicine of Lingnan (Southern China) and College of Pharmacy, Jinan University, Guangzhou 510632, China. , (China)
  • 4 State Key Laboratory of Research on Bioactivities and Clinical Applications of Medicinal Plants (CUHK), The Chinese University of Hong Kong, Shatin, N.T., Hong Kong, China. , (China)
Type
Published Article
Journal
Plants
Publisher
MDPI AG
Publication Date
Jan 15, 2021
Volume
10
Issue
1
Identifiers
DOI: 10.3390/plants10010161
PMID: 33467716
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Chinese herbal tea, also known as Liang Cha or cooling beverage, is popular in South China. It is regarded as a quick-fix remedy to relieve minor health problems. Hedyotis diffusa Willd. (colloquially Baihuasheshecao) is a common ingredient of cooling beverages. H. diffusa is also used to treat cancer and bacterial infections. Owing to the high demand for H. diffusa, two common adulterants, Hedyotis brachypoda (DC.) Sivar and Biju (colloquially Nidingjingcao) and Hedyotis corymbosa (L.) Lam. (colloquially Shuixiancao), are commonly encountered in the market. Owing to the close similarity of their morphological characteristics, it is difficult to differentiate them. Here, we sequenced the complete chloroplast genomes of the three species of Hedyotis using next-generation sequencing (NGS). By comparing the complete chloroplast genomes, we found that they are closely related in the subfamily Rubioideae. We also discovered that there are significant differences in the number and repeating motifs of microsatellites and complex repeats and revealed three divergent hotspots, rps16-trnQ intergenic spacer, ndhD and ycf1. By using these species-specific sequences, we propose new DNA barcoding markers for the authentication of H. diffusa and its two common adulterants.

Report this publication

Statistics

Seen <100 times