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Widely-tunable synchronisation-free picosecond laser source for multimodal CARS, SHG, and two-photon microscopy

Authors
  • Xu, Duanyang
  • Liang, Sijing
  • Xu, Lin
  • Bourdakos, Konstantinos N.
  • Johnson, Peter
  • Read, James
  • Price, Jonathan H. V.
  • Mahajan, Sumeet
  • Richardson, David J.
Type
Published Article
Journal
Biomedical Optics Express
Publisher
The Optical Society
Publication Date
Jan 26, 2021
Volume
12
Issue
2
Pages
1010–1019
Identifiers
DOI: 10.1364/BOE.411620
PMID: 33680556
PMCID: PMC7901310
Source
PubMed Central
License
Unknown

Abstract

We demonstrate a continuous wave (CW) seeded synchronization-free optical parametric amplifier (OPA) pumped by a picosecond, 1 µm laser and show its performance when used as a simple yet powerful source for label-free coherent anti-Stokes Raman scattering (CARS), concurrent second harmonic generation (SHG), and two-photon fluorescence microscopy in an epi-detection geometry. The average power level of above 175 mW, spectral resolution of 8 cm−1, and 2 ps pulse duration are well optimized for CARS microscopy in bio-science and bio-medical imaging systems. Our OPA is a much simpler setup than either the “gold-standard” laser and optical parametric oscillator (OPO) combination traditionally used for CARS imaging, or the more recently developed OPA systems pumped with femtosecond pulses [1]. Rapid and accurate tuning between resonances was achieved by changing the poled channels and temperature of the periodically-poled lithium niobate (PPLN) OPA crystal together with the OPA seed wavelength. The Pump-Stokes frequency detuning range fully covered the C-H stretching band used for the imaging of lipids. By enabling three multiphoton techniques using a compact, synchronization free laser source, our work paves the way for the translation of label-free multi-photon microscopy imaging from biomedical research to an imaging based diagnostic tool for use in the healthcare arena.

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