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Whole-genome sequencing of Staphylococcus aureus strain RN4220, a key laboratory strain used in virulence research, identifies mutations that affect not only virulence factors but also the fitness of the strain.

Authors
  • Nair, Dhanalakshmi
  • Memmi, Guido
  • Hernandez, David
  • Bard, Jonathan
  • Beaume, Marie
  • Gill, Steven
  • Francois, Patrice
  • Cheung, Ambrose L
Type
Published Article
Journal
Journal of Bacteriology
Publisher
American Society for Microbiology
Publication Date
May 01, 2011
Volume
193
Issue
9
Pages
2332–2335
Identifiers
DOI: 10.1128/JB.00027-11
PMID: 21378186
Source
Medline
License
Unknown

Abstract

Staphylococcus aureus RN4220, a cloning intermediate, is sometimes used in virulence, resistance, and metabolic studies. Using whole-genome sequencing, we showed that RN4220 differs from NCTC8325 and contains a number of genetic polymorphisms that affect both virulence and general fitness, implying a need for caution in using this strain for such studies.

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