Affordable Access

deepdyve-link
Publisher Website

When GIS zooms in: spatio-genetic maps of multipaternity in Armadillidium vulgare.

Authors
  • Bech, Nicolas1
  • Depeux, Charlotte2
  • Durand, Sylvine2
  • Debenest, Catherine2
  • Lafitte, Alexandra2
  • Beltran-Bech, Sophie2
  • 1 UMR CNRS 7267; Laboratoire Écologie and Biologie des Interactions; équipe Écologie, Évolution, Symbiose; Bâtiment B8-B35. 5, rue Albert Turpain TSA 51106, 86073, POITIERS CEDEX 9, France. [email protected] , (France)
  • 2 UMR CNRS 7267; Laboratoire Écologie and Biologie des Interactions; équipe Écologie, Évolution, Symbiose; Bâtiment B8-B35. 5, rue Albert Turpain TSA 51106, 86073, POITIERS CEDEX 9, France. , (France)
Type
Published Article
Journal
Genetica
Publisher
Springer-Verlag
Publication Date
Dec 01, 2017
Volume
145
Issue
6
Pages
503–512
Identifiers
DOI: 10.1007/s10709-017-9992-8
PMID: 28932924
Source
Medline
Keywords
License
Unknown

Abstract

Geographic information system (GIS) tools are designed to illustrate, analyse and integrate geographic or spatial data, usually on a macroscopic scale. By contrast, genetic tools focus on a microscopic scale. Because in reality, landscapes have no predefined scale, our original study aims to develop a new approach, combining both cartographic and genetic approaches to explore microscopic landscapes. For this, we focused on Armadillidium vulgare, a terrestrial isopod model in which evolutionary pressures imposed by terrestrial life have led to the development of internal fertilisation and, consequently, to associated physiological changes. Among these, the emergence of internal receptacles, found in many taxa ranging from mammals to arthropods, allowed females to store sperm from several partners, enabling multipaternity. Among arthropods, terrestrial isopods like the polygynandrous A. vulgare present a female structure, the marsupium, in which fertilised eggs migrate and develop into mancae (larval stage). To test our innovative combined approach, we proposed different males to four independent females, and at the end of incubation in the marsupium, we mapped (using GIS methods) and genotyped (using 12 microsatellite markers) all the incubated mancae. This methodology permitted to obtain spatio-genetic maps describing heterozygosity and spatial distribution of mancae and of multipaternity within the marsupial landscape. We discussed the interest of this kind of multidisciplinary approach which could improve in this case our understanding of sexual selection mechanisms in this terrestrial crustacean. Beyond the interesting model-focused insights, the main challenge of this study was the transfer of GIS techniques to a microscopic scale and our results appear so as pioneers rendering GIS tools available for studies involving imagery whatever their study scale.

Report this publication

Statistics

Seen <100 times