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Water-soluble conjugated polymers for fluorescent-enzyme assays.

Authors
  • Feng, Fude
  • Liu, Libing
  • Yang, Qiong
  • Wang, Shu
Type
Published Article
Journal
Macromolecular rapid communications
Publication Date
Aug 17, 2010
Volume
31
Issue
16
Pages
1405–1421
Identifiers
DOI: 10.1002/marc.201000020
PMID: 21567544
Source
Medline
License
Unknown

Abstract

Enzyme assays are receiving more and more research and application interest because of the rapidly increasing demands of clinical diagnosis, environmental analysis, drug discovery, and molecular biology. Water-soluble light-harvesting conjugated polymers (CPs) coordinate the action of a large number of absorbing units to afford an amplified fluorescence signal, which makes them useful as optical platforms in highly sensitive chemical and biological sensors. This Feature Article highlights recent developments of water-soluble CPs for fluorescent assays of enzymes. Different signal transduction mechanisms, such as electron transfer, fluorescence resonance energy transfer (FRET), and aggregation or conformation changes of CPs, are employed in these assays according to the dissimilar nature of enzymes. Potential challenges and future research directions in these approaches based on CPs are also discussed.

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