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Wasted Ganoderma tsugae Derived Chitosans for Smear Layer Removal in Endodontic Treatment

Authors
  • Huang, Sheng-Tung1
  • Teng, Nai-Chia
  • Wang, Hsin-Hui
  • Hsieh, Sung-Chih
  • Yang, Jen-Chang1, 2, 3, 4
  • 1 Research Center of Biomedical Device, Taipei Medical University, Taipei 110-52, Taiwan
  • 2 Graduate Institute of Nanomedicine and Medical Engineering, College of Biomedical Engineering, Taipei Medical University, Taipei 110-31, Taiwan
  • 3 International Ph.D. Program in Biomedical Engineering, College of Biomedical Engineering, Taipei Medical University, Taipei 110-31, Taiwan
  • 4 Research Center of Digital Oral Science and Technology, Taipei Medical University, Taipei, 110-52, Taiwan
Type
Published Article
Journal
Polymers
Publisher
MDPI AG
Publication Date
Nov 01, 2019
Volume
11
Issue
11
Identifiers
DOI: 10.3390/polym11111795
PMID: 31683976
PMCID: PMC6918306
Source
PubMed Central
Keywords
License
Green

Abstract

The objective of this study is to investigate the synergistic effects of acid etching and metal-ion chelation in dental smear layer removal using wasted Ganoderma tsugae derived chitosans. The wasted Ganoderma tsugae fruiting body was used to prepare both acid-soluble fungal chitosan (FCS) and alkali-soluble polysaccharide (ASP). To explore the effective irrigant concentration for smear layer removal, a chelating effect on ferrous ions was conducted. Specimens of various concentrations of EDTA, citric acid, and polysaccharide solutions were reacted with FerroZine™ then the absorbance was examined at 562 nm by a UV-visible spectrophotometer to calculate their metal chelating capability. Twenty extracted premolars were instrumented and individually soaked in the solutions of 15 wt% EDTA, 10 wt% citric acid, 0.04 wt% ASP, 0.04 wt% FCS, and normal saline were randomly divided into five groups (N=4). Next, each tooth was cleaved longitudinally and examined by scanning electron microscopy (SEM) to assay the effectiveness of smear layer removal. The chelating capability for EDTA, FCS, and ASP showed no significant difference over the concentration of 0.04 wt% ( p > 0.05). The SEM results showed that 0.04 wt% FCS solution was effective in smear layer removal along the canal wall. These results indicated that Ganoderma tsuage derived FCS in acid solutions could be a potential alternative as a root canal irrigant solution due to its synergistic effect.

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