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VP1 codon deoptimization and high-fidelity substitutions in 3D polymerase as potential vaccine strategies for eliciting immune responses against enterovirus A71

Authors
  • Hsieh, Wen-Sheng
  • Chao, Chiao-Hsuan
  • Shen, Chun-Yu
  • Cheng, Dayna
  • Huang, Sheng-Wen
  • Wang, Ya-Fang
  • Chen, Chien-Chin
  • Chen, Shun-Hua
  • Hsu, Li-Jin
  • Wang, Jen-Ren
Type
Published Article
Journal
Journal of Virology
Publisher
American Society for Microbiology
Publication Date
Jan 04, 2024
Volume
98
Issue
1
Identifiers
DOI: 10.1128/jvi.01558-23
Source
ASM Journals
Keywords
License
Yellow

Abstract

EV-A71 can cause severe neurological diseases and cause death in young children. Here, we report the development of synthetic rgEV-A71s with the combination of codon deoptimization and high-fidelity (HF) substitutions that generate genetically stable reverse genetics (rg) viruses as potential attenuated vaccine candidates. Our work provides insight into the development of low-virulence candidate vaccines through a series of viral genetic editing for maintaining antigenicity and genome stability and suggests a strategy for the development of an innovative next-generation vaccine against EV-A71.

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