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Voltammetric aptasensor for thrombin by using a gold microelectrode modified with graphene oxide decorated with silver nanoparticles

Authors
  • Qin, Bei1, 2
  • Yang, Kuan1, 3
  • 1 Xi’an Medical University, Department of Pharmacy, Xi’an, 710021, People’s Republic of China , Xi’an (China)
  • 2 Xi ’an Jiaotong University, Faculty of Science, Xi’an, 710049, People’s Republic of China , Xi’an (China)
  • 3 Shaanxi University of Science and Technology, School of Food and Biological Engineering, Xi’an, 710021, People’s Republic of China , Xi’an (China)
Type
Published Article
Journal
Microchimica Acta
Publisher
Springer-Verlag
Publication Date
Aug 09, 2018
Volume
185
Issue
9
Identifiers
DOI: 10.1007/s00604-018-2924-1
Source
Springer Nature
Keywords
License
Yellow

Abstract

A sensitive electrochemical aptasensor was developed for the determination of thrombin. It is based on signal amplification by using graphene oxide (GO) decorated with silver nanoparticles (AgNP) and placed on a microelectrode. The graphene oxide decorated with silver nanoparticles (AgNP-GO) was synthesized by chemical reduction and then modified with a thrombin-binding aptamer 1 (TBA1; a 29-mer) carrying a 3′-terminal thiol group. It was immobilized on the AgNP-GO by self-assembled monolayer technique to form the signaling probe (TBA1-AgNP-GO). A capture thrombin-binding aptamer 2 (TBA-2; a 15-mer) carrying a 3′-terminal thiol group was self-assembled onto the surface of the gold electrode. After binding of thrombin and the signaling probe, a sandwich of type TBA2-thrombin-TBA1-AgNP-GO is formed. The square wave voltammetric signal of the AgNP is then recorded. The electrochemical oxidation signal (measured at 0.21 V vs. Ag/AgCl) increases linearly with the concentration of thrombin in the range from 0.05 nmol·L−1 to 5 nmol·L−1 with a detection limit of 0.03 nmol·L−1 (at S/N = 3). The aptasensor is highly specific and not affected by other proteins. It was successfully applied to the determination of thrombin in spiked human serum samples. Graphical abstractA sensitive electrochemical aptasensor for thrombin was developed based on the signal amplification of silver nanoparticles decorated graphene oxide at an ultramicroelectrode

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