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Voltage-gated sodium channel expressed in cultured human smooth muscle cells: involvement of SCN9A.

Authors
  • Jo, Taisuke
  • Nagata, Taiji
  • Iida, Haruko
  • Imuta, Hiroyuki
  • Iwasawa, Kuniaki
  • Ma, Ji
  • Hara, Kei
  • Omata, Masao
  • Nagai, Ryozo
  • Takizawa, Hajime
  • Nagase, Takahide
  • Nakajima, Toshiaki
Type
Published Article
Journal
FEBS Letters
Publisher
Wiley (John Wiley & Sons)
Publication Date
Jun 04, 2004
Volume
567
Issue
2-3
Pages
339–343
Identifiers
PMID: 15178348
Source
Medline
License
Unknown

Abstract

Voltage-gated Na(+) channel (I(Na)) is expressed under culture conditions in human smooth muscle cells (hSMCs) such as coronary myocytes. The aim of this study is to clarify the physiological, pharmacological and molecular characteristics of I(Na) expressed in cultured hSMCs obtained from bronchus, main pulmonary and coronary artery. I(Na), was recorded in these hSMCs and inhibited by tetrodotoxin (TTX) with an IC(50) value of approximately 10 nM. Reverse transcriptase/polymerase chain reaction (RT-PCR) analysis of mRNA showed the prominent expression of transcripts for SCN9A, which was consistent with the results of real-time quantitative RT-PCR. These results provide novel evidence that TTX-sensitive Na(+) channel expressed in cultured hSMCs is mainly composed of Na(v)1.7.

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