Affordable Access

deepdyve-link
Publisher Website

In vivo quantitative magnetization transfer imaging correlates with histology during de- and remyelination in cuprizone-treated mice.

Authors
  • Turati, Laura
  • Moscatelli, Marco
  • Mastropietro, Alfonso
  • Dowell, Nicholas G
  • Zucca, Ileana
  • Erbetta, Alessandra
  • Cordiglieri, Chiara
  • Brenna, Greta
  • Bianchi, Beatrice
  • Mantegazza, Renato
  • Cercignani, Mara
  • Baggi, Fulvio
  • Minati, Ludovico
Type
Published Article
Journal
NMR in Biomedicine
Publisher
Wiley (John Wiley & Sons)
Publication Date
Mar 01, 2015
Volume
28
Issue
3
Pages
327–337
Identifiers
DOI: 10.1002/nbm.3253
PMID: 25639498
Source
Medline
Keywords
License
Unknown

Abstract

The pool size ratio measured by quantitative magnetization transfer MRI is hypothesized to closely reflect myelin density, but their relationship has so far been confirmed mostly in ex vivo conditions. We investigate the correspondence between this parameter measured in vivo at 7.0 T, with Black Gold II staining for myelin fibres, and with myelin basic protein and beta-tubulin immunofluorescence in a hybrid longitudinal study of C57BL/6 and SJL/J mice treated with cuprizone, a neurotoxicant causing relatively selective myelin loss followed by spontaneous remyelination upon treatment suspension. Our results confirm that pool size ratio measurements correlate with myelin content, with the correlation coefficient depending on strain and staining method, and demonstrate the in vivo applicability of this MRI technique to experimental mouse models of multiple sclerosis.

Report this publication

Statistics

Seen <100 times