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In vivo identification of the transforming gene product of simian sarcoma virus.

Authors
  • Robbins, K C
  • Devare, S G
  • Reddy, E P
  • Aaronson, S A
Type
Published Article
Journal
Science (New York, N.Y.)
Publication Date
Dec 10, 1982
Volume
218
Issue
4577
Pages
1131–1133
Identifiers
PMID: 6293053
Source
Medline
License
Unknown

Abstract

Simian sarcoma virus (SSV) deletion mutants were constructed from a molecular clone containing the entire infectious provirus. Transfection analysis of these mutants localized the SSV transforming gene to a small region of the viral genome encompassing its cell-derived sequence (v-sis). Antiserum to a peptide synthesized on the basis of the predicted amino acid sequence of the SSV transforming gene detected a 28,000-dalton protein that was specifically expressed in SSV transformed cells and that corresponded in size to that predicted from the v-sis coding sequence. The v-sis gene product designated p28sis was not a phosphoprotein, nor did it possess detectable protein kinase activity. These findings distinguish p28sis from a number of other retroviral onc proteins.

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