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In vivo fluorescent imaging of the mouse retina using adaptive optics.

Authors
  • Biss, David P
  • Sumorok, Daniel
  • Burns, Stephen A
  • Webb, Robert H
  • Zhou, Yaopeng
  • Bifano, Thomas G
  • Côté, Daniel
  • Veilleux, Israel
  • Zamiri, Parisa
  • Lin, Charles P
Type
Published Article
Journal
Optics letters
Publication Date
Mar 15, 2007
Volume
32
Issue
6
Pages
659–661
Identifiers
PMID: 17308593
Source
Medline
License
Unknown

Abstract

In vivo imaging of the mouse retina using visible and near infrared wavelengths does not achieve diffraction-limited resolution due to wavefront aberrations induced by the eye. Considering the pupil size and axial dimension of the eye, it is expected that unaberrated imaging of the retina would have a transverse resolution of 2 microm. Higher-order aberrations in retinal imaging of human can be compensated for by using adaptive optics. We demonstrate an adaptive optics system for in vivo imaging of fluorescent structures in the retina of a mouse, using a microelectromechanical system membrane mirror and a Shack-Hartmann wavefront sensor that detects fluorescent wavefront.

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