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In vivo degradation of secreted fusion proteins by the Escherichia coli outer membrane protease OmpT.

Authors
  • Baneyx, F
  • Georgiou, G
Type
Published Article
Journal
Journal of bacteriology
Publication Date
Jan 01, 1990
Volume
172
Issue
1
Pages
491–494
Identifiers
PMID: 2403549
Source
Medline
License
Unknown

Abstract

The Escherichia coli outer membrane protease OmpT (protease VII) has been shown to degrade several proteins in vitro, but its function in vivo is uncertain. We demonstrate that OmpT participates in the degradation of a fusion protein secreted into the periplasmic space. A strain with mutations in degP (K.L. Strauch and J. Beckwith, Proc. Natl. Acad. Sci. USA 85:1576-1580, 1988) and ompT exhibits a cumulative decrease in protein degradation and should be useful for the expression of proteolytically sensitive secreted proteins.

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