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In vivo biomolecular imaging of zebrafish embryos using confocal Raman spectroscopy

Authors
  • Hogset, H
  • Horgan, C
  • Bergholt, M
  • Armstrong, J
  • Torraca, V
  • Chen, Q
  • Keane, TJ
  • Bugeon, L
  • Dallman, M
  • Mostowy, S
  • Stevens, M
Publication Date
Oct 28, 2020
Source
UPCommons. Portal del coneixement obert de la UPC
License
Unknown

Abstract

Zebrafish embryos provide a unique opportunity to visualize complex biological processes, yet conventional imaging modalities are unable to access intricate biomolecular information without compromising the integrity of the embryos. Here, we report the use of confocal Raman spectroscopic imaging for the visualization and multivariate analysis of biomolecular information extracted from unlabeled zebrafish embryos. We outline broad applications of this method in: (i) visualizing the biomolecular distribution of whole embryos in three dimensions, (ii) resolving anatomical features at subcellular spatial resolution, (iii) biomolecular profiling and discrimination of wild type and ΔRD1 mutant Mycobacterium marinum strains in a zebrafish embryo model of tuberculosis and (iv) in vivotemporal monitoring of the wound response in living zebrafish embryos.Overall, this study demonstrates the application of confocal Raman spectroscopic imaging for the comparative bimolecular analysis in fully intact and living zebrafish embryos.

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