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In vitro and in vivo characterization of a novel H1N1/2009 influenza virus reassortant with an NS gene from a highly pathogenic H5N1 virus, isolated from a human

Authors
  • Liu, Shengbing1
  • Zhang, Liangyan2
  • Yao, Zhidong2
  • Xing, Li2
  • Liu, Kun2
  • 1 Jiaxing University, Jiaxing, Zhejiang, 314001, China , Jiaxing (China)
  • 2 Beijing Institute of Microbiology and Epidemiology, No. 20 Dongda Street, Beijing, 100071, China , Beijing (China)
Type
Published Article
Journal
Archives of Virology
Publisher
Springer-Verlag
Publication Date
May 18, 2017
Volume
162
Issue
9
Pages
2633–2642
Identifiers
DOI: 10.1007/s00705-017-3408-z
Source
Springer Nature
Keywords
License
Yellow

Abstract

The triple-reassortant H1N1/2009 influenza A virus, which caused the first influenza pandemic of the 21st century, is generally associated with mild disease and a relatively low mortality rate comparable to that of seasonal influenza virus outbreaks. There is a growing concern about the potential for reassortment between the low-mortality H1N1/2009 and other high-mortality influenza viruses. Here, we describe and characterize a novel reassortant H1N1/2009 influenza virus, isolated from a human sample, that contained an NS gene from a highly pathogenic H5N1 virus. We evaluated the effect of the acquired NS gene on viral virulence both in vitro and in vivo and found that the novel NS-reassorted influenza virus replicated well in different cell lines and several organs of BALB/c mice without prior adaption and induced a cytokine imbalance. Therefore, there is a continued risk for further reassortment of the H1N1/2009 virus, and therefore, systematic surveillance should be enhanced to prepare for the next possible pandemic.

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