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In vitro tooth-shaped scaffold construction by mimicking late bell stage.

Authors
  • TaŞli, Pakize Neslihan1
  • YalÇin Ülker, Gül Merve2
  • Cumbul, Alev3
  • Uslu, Ünal3
  • Yilmaz, Şahin1
  • Bozkurt, Batuhan Turhan1
  • Şahİn, Fikrettin1
  • 1 Department of Genetic and Bioengineering, Faculty of Engineering and Architecture, Yeditepe University, İstanbul Turkey. , (Turkey)
  • 2 Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, İstanbul Okan University, İstanbul Turkey. , (Turkey)
  • 3 Department of Histology and Embryology, Faculty of Medicine, Yeditepe University, İstanbul Turkey. , (Turkey)
Type
Published Article
Journal
Turkish journal of biology = Turk biyoloji dergisi
Publication Date
Jan 01, 2020
Volume
44
Issue
5
Pages
315–326
Identifiers
DOI: 10.3906/biy-2002-19
PMID: 33110369
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Neogenesis of osseous and ligamentous interfacial structures is essential for the regeneration of large oral or craniofacial defects. However, current treatment strategies are inadequate in renewing supporting tissues of teeth after trauma, chronic infections or surgical resection. Combined use of 3D scaffolds with stem cells became a promising treatment option for these injuries. Matching different scaffolding materials with different tissues can induce the correct cytokines and the differentiation of cells corresponding to that particular tissue. In this study, a hydroxyapatite (HA) based scaffold was used together with human adipose stem cells (hASCs), human bone marrow stem cells (hBMSCs) and gingival epithelial cells to mimic human tooth dentin-pulp-enamel tissue complexes and model an immature tooth at the late bell stage in vitro. Characteristics of the scaffold were determined via SEM, FTIR, pore size and density measurements. Changes in gene expression, protein secretions and tissue histology resulting from cross-interactions of different dental tissues grown in the system were shown. Classical tooth tissues such as cementum, pulp and bone like tissues were formed within the scaffold. Our study suggests that a HA-based scaffold with different cell lineages can successfully mimic early stages of tooth development and can be a valuable tool for hard tissue engineering. Copyright © 2020 The Author(s).

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