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In vitro systems for the reconstitution of snRNP and protein nuclear import.

Authors
  • Dingwall, C
  • Palacios, I
Type
Published Article
Journal
Methods in Cell Biology
Publisher
Elsevier BV
Publication Date
Jan 01, 1998
Volume
53
Pages
517–543
Identifiers
PMID: 9348523
Source
Medline
License
Unknown

Abstract

In this chapter we have presented the most recent methods for the preparation of cell extracts and recombinant protein factors for the reconstitution of nuclear protein and snRNP import in vitro. In addition, we have discussed methods available for the quantitation of the level of import into nuclei. Accurate quantitation is particularly important when the effects of inhibitors are to be compared and when estimates of nuclear import rate are required.

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