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In vitro NMR characterization of mammalian myocardium: effect of specimen integrity on relaxation times.

Authors
  • Scholz, T D
  • Fleagle, S R
  • Skorton, D J
Type
Published Article
Journal
Magnetic resonance in medicine
Publication Date
Sep 01, 1989
Volume
11
Issue
3
Pages
367–370
Identifiers
PMID: 2674601
Source
Medline
License
Unknown

Abstract

We systematically evaluated three different methods of preparing canine myocardium for NMR relaxometry: using whole specimens, specimens sliced into approximately 2-mm3 pieces, and specimens minced into pieces less than 2 mm3. NMR relaxation times were determined at 20 MHz. T1 relaxation time and water content were not different among the three methods. However, T2 values differed significantly between whole and sliced samples (mean +/- SEM = 51.2 +/- 2.2 ms (whole) vs 54.3 +/- 2.7 ms (sliced): P less than 0.05). Minced myocardium showed a similar increase that was not statistically significant. We conclude that tissue preparation methods must be controlled when performing NMR relaxometry studies.

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