Affordable Access

deepdyve-link
Publisher Website

An In Vitro Model of Cellular Quiescence in Primary Human Dermal Fibroblasts.

Authors
  • Mitra, Mithun1, 2
  • Ho, Linda D1
  • Coller, Hilary A3, 4
  • 1 Department of Molecular, Cell and Developmental Biology, 5145 Terasaki Life Science Building, 610 Charles E. Young Drive E., University of California, Los Angeles, 90095-7329, USA.
  • 2 Department of Biological Chemistry, David Geffen School of Medicine, Los Angeles, CA, 90095-7329, USA.
  • 3 Department of Molecular, Cell and Developmental Biology, 5145 Terasaki Life Science Building, 610 Charles E. Young Drive E., University of California, Los Angeles, 90095-7329, USA. [email protected]
  • 4 Department of Biological Chemistry, David Geffen School of Medicine, Los Angeles, CA, 90095-7329, USA. [email protected]
Type
Published Article
Journal
Methods in molecular biology (Clifton, N.J.)
Publication Date
Jan 01, 2018
Volume
1686
Pages
27–47
Identifiers
DOI: 10.1007/978-1-4939-7371-2_2
PMID: 29030810
Source
Medline
Keywords
License
Unknown

Abstract

Cellular quiescence is a reversible mode of cell cycle exit that allows cells and organisms to withstand unfavorable stress conditions. The factors that underlie the entry, exit, and maintenance of the quiescent state are crucial for understanding normal tissue development and function as well as pathological conditions such as chronic wound healing and cancer. In vitro models of quiescence have been used to understand the factors that contribute to quiescence under well-controlled experimental conditions. Here, we describe an in vitro model of quiescence that is based on neonatal human dermal fibroblasts. The fibroblasts are induced into quiescence by antiproliferative signals, contact inhibition, and serum-starvation (mitogen withdrawal). We describe the isolation of fibroblasts from skin, methods for inducing quiescence in isolated fibroblasts, and approaches to manipulate the fibroblasts in proliferating and quiescent states to determine critical regulators of quiescence.

Report this publication

Statistics

Seen <100 times