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In vitro metabolism of 17beta-estradiol by human liver tissue.

Authors
  • Hobkirk, R
  • Mellor, J D
  • Nilsen, M
Type
Published Article
Journal
Canadian journal of biochemistry
Publication Date
Aug 01, 1975
Volume
53
Issue
8
Pages
903–906
Identifiers
PMID: 1181008
Source
Medline
License
Unknown

Abstract

17beta-[6,7- 3H]Estradiol was incubated with adult human liver slices in Krebs-Ringer phosphate buffer containing glucose. Of the identified 3H recovered, 51-76 percent consisted of estrone-3-sulfate (E13S) and 17 beta-estradiol-3-sulfate (E23S). E13S was the main metabolite and was found in both tissue and medium. E23S was present only in the medium. Minor amounts of estrogen glucuronides were formed. When a human liver homogenate was incubated with [3H]E2 in a medium fortified with excess uridine diphosphate glucuronic acid only some 4 percent of conjugation with glucuronic acid was observed. It is suggested that human liver favors sulfurylation as the conjugating mechanism for E2 and E1.

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