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In vitro measurements of heat production rate of rat kidney. A microcalorimetric study.

Authors
Type
Published Article
Journal
Research in experimental medicine. Zeitschrift für die gesamte experimentelle Medizin einschliesslich experimenteller Chirurgie
Publication Date
Volume
190
Issue
3
Pages
193–201
Identifiers
PMID: 2367744
Source
Medline

Abstract

In the present study heat production rate (P) of rat kidney tissue in vitro has been measured with a microcalorimetric technique. Cortex slices (0.3-0.7 mm) were incubated in a balanced electrolyte solution or in medium E-199. The metabolic activities in these different media were found to be 0.87 microW/mg and 0.98 microW/mg, respectively, determined by using 5-mg slices after 1 h of incubation (P1h). Medulla slices with a thickness of 0.4 mm demonstrated a lower P, i.e., 0.65 microW/mg. The heat output was not influenced by the thickness of those slices, ranging from 0.3 to 0.7 mm. However, an increase in the wet weight of the tissue sample was accompanied by a reduced P, expressed as power per milligram wet weight. The magnitude of this crowding phenomenon decreased as the time of incubation increased. Storage of tissue for 3.5 h prior to measurement caused a 6-7% reduction of P. This study indicates that the microcalorimetric technique is a sensitive method for measuring metabolism in small kidney biopsies.

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