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In vitro isolation and characterisation of a bovine Neospora species in Japan.

Authors
  • Yamane, I
  • Kokuho, T
  • Shimura, K
  • Eto, M
  • Shibahara, T
  • Haritani, M
  • Ouchi, Y
  • Sverlow, K
  • Conrad, P A
Type
Published Article
Journal
Research in Veterinary Science
Publisher
Elsevier
Publication Date
Jan 01, 1997
Volume
63
Issue
1
Pages
77–80
Identifiers
PMID: 9368961
Source
Medline
License
Unknown

Abstract

Eleven aborted bovine fetuses and five calves suspected as having neosporosis were necropsied and tissues from these animals were inoculated into bovine cardiopulmonary aortic endothelial cells and monkey kidney cells and maintained at 37 degrees C with 5 per cent CO2. Neospora tachyzoites were observed in one cell 49 days after inoculation. The isolated parasite (JPA1) was morphologically identical to the previously reported bovine Neospora species (BPA1) and confirmed by its strong antigenic reactivity with bovine control antisera to Neospora species and its lack of reactivity with Toxoplasma gondii and Sarcocystis cruzi antisera. This is the first bovine Neospora species isolate in Asia and further studies with this isolate are now expected.

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