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In vitro investigation of antioxidant and antihemolytic activities of three Lamiaceae species from Morocco

Authors
  • Hmidani, Abdelbassat1, 1
  • Bouhlali, Eimad dine Tariq2
  • Ajebli, Mohammed1
  • Khouya, Tarik1
  • Benlyas, Mohamed1
  • Alem, Chakib1
  • 1 Moulay Ismail University, Zitoune, Meknes, BP, 11201, Morocco , Meknes (Morocco)
  • 2 National Institute of Agronomic Research Regional Center of Errachidia, Errachidia, Morocco , Errachidia (Morocco)
Type
Published Article
Journal
Beni-Suef University Journal of Basic and Applied Sciences
Publisher
Springer Berlin Heidelberg
Publication Date
Apr 17, 2021
Volume
10
Issue
1
Identifiers
DOI: 10.1186/s43088-021-00116-9
Source
Springer Nature
Keywords
License
Green

Abstract

BackgroundLkhzama (Lavandula officinalis), Mard-doch (Origanum majorana), and Lahbak (Ocimum basilicum) are aromatic and medicinal plants widely used in Moroccan folk medicine as a treatment for numerous diseases including liver diseases, rheumatism, and diabetes. This study was undertaken to examine the antioxidant and antihemolytic activities of the aqueous extracts of these plants. The antioxidant activity was evaluated using three in vitro tests: DPPH (2,2-diphenyl-1-picryl-hydrazyl-hydrate) radical scavenging activity, FRAP (ferric reducing antioxidant power assay), and ABTS (2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging assay. The antihemolytic activity of plant extracts was evaluated against AAPH (2,2′-azobis(2-amidino-propane) dihydrochloride)-induced erythrocyte hemolysis.ResultsOur findings showed that all plant extracts displayed significant antioxidant and antihemolytic effects. In fact, among the studied plant extracts, the highest antioxidant power was recorded in Origanum majorana, based on DPPH (IC50 = 12.29 μg/mL), ABTS (226.13 μmol TE/g DW), and FRAP (477.82 μmol TE/g DW) assays. Moreover, the same plant also showed the best membrane protective effect (269.55%). Whereas, Ocimum basilicum exhibited the lowest antioxidant activity using DPPH (IC50 = 42.85 μg/mL), ABTS (IC50 = 226.13 μmol TE/g DW), and FRAP (IC50 = 172.84 μmol TE/g DW) and, thus, the lowest membrane protective effect (182.70%).ConclusionThis result supports the use of these plants in folk medicine for preventing and treating many diseases, especially those related to oxidative stress.

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