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In vitro growth of cytotoxic human lymphocytes. II. Use of T cell growth factor (TCGF) to clone human T cells.

Authors
Type
Published Article
Journal
Journal of immunology (Baltimore, Md. : 1950)
Publication Date
Volume
124
Issue
6
Pages
2972–2978
Identifiers
PMID: 6989911
Source
Medline

Abstract

Soft agar and limiting dilution techniques utilizing human T cell growth factor (TCGF) have been developed to clone human lymphoid cells with high cloning efficiency. TCGF has been used for the continued growth and expansion of lymphoid populations derived from single T cells, and the cytotoxic specificity of individual clones has been repeatedly examined. An analysis of clones derived from human lymphocytes sensitized to allogeneic determinants in vitro reveals significant polymorphism in lytic recognition when tested against HLA typed targets. This pattern of reactivity remained constant over the 2 months of continuous culture. These methods should be capable of dissecting and analyzing the fine specificity of the T cell response.

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