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In Vitro Glucuronidation of Ochratoxin A by Rat Liver Microsomes

Authors
  • han, zheng
  • tangni, emmanuel
  • di mavungu, josé
  • vanhaecke, lynn
  • de saeger, sarah
  • aibo, wu
  • callebaut, alfons
Publication Date
Dec 18, 2013
Identifiers
DOI: 10.3390/toxins5122671
OAI: oai:mdpi.com:/2072-6651/5/12/2671/
Source
MDPI
Keywords
Language
English
License
Green
External links

Abstract

Ochratoxin A (OTA), one of the most toxic mycotoxins, can contaminate a wide range of food and feedstuff. To date, the data on its conjugates via glucuronidation request clarification and consolidation. In the present study, the combined approaches of ultra high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS), UHPLC-Orbitrap-high resolution mass spectrometry (HRMS) and liquid chromatography-multiple stage mass spectrometry (LC-MSn) were utilized to investigate the metabolic profile of OTA in rat liver microsomes. Three conjugated products of OTA corresponding to amino-, phenol- and acyl-glucuronides were identified, and the related structures were confirmed by hydrolysis with β-glucuronidase. Moreover, OTA methyl ester, OTα and OTα-glucuronide were also found in the reaction solution. Based on these results, an in vitro metabolic pathway of OTA has been proposed for the first time.

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