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In Vitro Evaluation of the Apoptosis Function in Human Activated T Cells

Authors
Type
Published Article
Journal
Methods in Molecular Biology
Publication Date
Jul 05, 2017
Volume
1557
Pages
33–40
Identifiers
DOI: 10.1007/978-1-4939-6780-3_4
PMID: 28078580
Source
USPC - SET - SVS
License
White

Abstract

The apoptosis function can be monitored on human lymphocytes by quantifying the induced-death upon apoptotic stimuli involving either the extrinsic or the intrinsic pathway on in vitro activated T cells. Her, we describe an in vitro assay allowing the monitoring of three different apoptosis pathways: (1) the FAS-induced pathway, (2) the activation-induced cell death (AICD), and (3) the death induced by starvation of the cells, called activated-cell autonomous death (ACAD).

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